Colloidal gold nanoparticles (AuNPs) have been considered an established advanced tool in biomedicine thanks to their physicochemical properties combined with nanoscale size ideal for the interrogation of biological systems. However, such properties are believed to be a possible major cause of “unsafety” of these materials. For this reason, increasing attention has been due to assess how AuNPs affect cell behaviour in cultures. In the present work, we investigate the effects of PMA polymer-coated Au@PMA PEGylated (8.9 ± 0.2 nm) or not (6.6 ± 0.6 nm) on HUVECs and macrophages, which are model cell types likely to interact with Au@PMA after systemic administration in vivo, using a multiparametric approach. Testing different NPs concentrations and incubation times, we analysed the effect of such NPs on cell viability, oxidative stress, inflammatory processes, and cell uptake. Our data suggested that Au@PMA reduced the cell viability mostly through oxidative stress and TNF-α production after the uptake by HUVECs and macrophages, respectively. PEGylation conferred improved biocompatibility to Au@PMA in particular, no significant effects on any parameter tested could be observed at a concentration of 20 µg mL−1. This approach allowed us to explore different aspects of cell-NPs interaction and to suggest that these NPs could be potentially used for the in vivo studies.

Orlando, A., Colombo, M., Prosperi, D., Corsi, F., Panariti, A., Rivolta, I., et al. (2016). Evaluation of gold nanoparticles biocompatibility: a multiparametric study on cultured endothelial cells and macrophages. JOURNAL OF NANOPARTICLE RESEARCH, 18(3), 1-12 [10.1007/s11051-016-3359-4].

Evaluation of gold nanoparticles biocompatibility: a multiparametric study on cultured endothelial cells and macrophages

ORLANDO, ANTONINA
Primo
;
COLOMBO, MIRIAM
Secondo
;
PROSPERI, DAVIDE;PANARITI, ALICE LUCIA;RIVOLTA, ILARIA;MASSERINI, MASSIMO ERNESTO
Penultimo
;
CAZZANIGA, EMANUELA
2016

Abstract

Colloidal gold nanoparticles (AuNPs) have been considered an established advanced tool in biomedicine thanks to their physicochemical properties combined with nanoscale size ideal for the interrogation of biological systems. However, such properties are believed to be a possible major cause of “unsafety” of these materials. For this reason, increasing attention has been due to assess how AuNPs affect cell behaviour in cultures. In the present work, we investigate the effects of PMA polymer-coated Au@PMA PEGylated (8.9 ± 0.2 nm) or not (6.6 ± 0.6 nm) on HUVECs and macrophages, which are model cell types likely to interact with Au@PMA after systemic administration in vivo, using a multiparametric approach. Testing different NPs concentrations and incubation times, we analysed the effect of such NPs on cell viability, oxidative stress, inflammatory processes, and cell uptake. Our data suggested that Au@PMA reduced the cell viability mostly through oxidative stress and TNF-α production after the uptake by HUVECs and macrophages, respectively. PEGylation conferred improved biocompatibility to Au@PMA in particular, no significant effects on any parameter tested could be observed at a concentration of 20 µg mL−1. This approach allowed us to explore different aspects of cell-NPs interaction and to suggest that these NPs could be potentially used for the in vivo studies.
Articolo in rivista - Articolo scientifico
Cellular toxicity; Colloids; Endothelial cells; Gold nanoparticles; Health effects; Macrophages; Nanoparticle uptake; Oxidative stress;
Cellular toxicity; Colloids; Endothelial cells; Gold nanoparticles; Health effects; Macrophages; Nanoparticle uptake; Oxidative stress; Atomic and Molecular Physics, and Optics; Condensed Matter Physics; Modeling and Simulation; Chemistry (all); Materials Science (all); Bioengineering
English
2016
18
3
1
12
58
none
Orlando, A., Colombo, M., Prosperi, D., Corsi, F., Panariti, A., Rivolta, I., et al. (2016). Evaluation of gold nanoparticles biocompatibility: a multiparametric study on cultured endothelial cells and macrophages. JOURNAL OF NANOPARTICLE RESEARCH, 18(3), 1-12 [10.1007/s11051-016-3359-4].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/138700
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