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Fourier transform infrared (FTIR) spectroscopy is a useful tool for the structural characterization of insoluble protein assemblies, as it allows to obtain information on the protein secondary structures and on their intermolecular interactions. The protocols for FTIR spectroscopy and microspectroscopy measurements in transmission and attenuated total reflection modes will be presented and illustrated in the following examples: bacterial inclusion bodies, self-assembling peptides, thermal aggregates, and amyloid fibrils.

Natalello, A., Doglia, S. (2015). Insoluble protein assemblies characterized by fourier transform infrared spectroscopy. In E. García-Fruitós (a cura di), Insoluble Proteins : Methods and Protocols (pp. 347-369). Springer-Verlag New York Inc. [10.1007/978-1-4939-2205-5_20].

Insoluble protein assemblies characterized by fourier transform infrared spectroscopy

NATALELLO, ANTONINO
;DOGLIA, SILVIA MARIA
Ultimo
2015

Abstract

Fourier transform infrared (FTIR) spectroscopy is a useful tool for the structural characterization of insoluble protein assemblies, as it allows to obtain information on the protein secondary structures and on their intermolecular interactions. The protocols for FTIR spectroscopy and microspectroscopy measurements in transmission and attenuated total reflection modes will be presented and illustrated in the following examples: bacterial inclusion bodies, self-assembling peptides, thermal aggregates, and amyloid fibrils.
Capitolo o saggio
Amyloid; Fourier transform infrared spectroscopy; Hydrogel; Inclusion body; Microspectroscopy; Peptide scaffold; Protein aggregation; Secondary structures; Spectroscopy; β-sheet; Amyloid; Animals; Bacteria; Humans; Inclusion Bodies; Peptides; Protein Structure, Secondary; Spectroscopy, Fourier Transform Infrared; Molecular Biology; Genetics; Medicine (all)
English
Insoluble Proteins : Methods and Protocols
García-Fruitós, E
2015
9781493922048
1258
Springer-Verlag New York Inc.
347
369
Natalello, A., Doglia, S. (2015). Insoluble protein assemblies characterized by fourier transform infrared spectroscopy. In E. García-Fruitós (a cura di), Insoluble Proteins : Methods and Protocols (pp. 347-369). Springer-Verlag New York Inc. [10.1007/978-1-4939-2205-5_20].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/138698
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