Membranes and detergent-resistant membrane fractions isolated from human epidermoid carcinoma A431 cells after treatment with methyl-beta-cyclodextrin, a compound commonly used in pharmaceutical applications and in manipulation of membrane cholesterol content, display thermotropic transitions at about 15 degrees C and above 37 degrees C, respectively, when analyzed by differential scanning calorimetry. The transitions, absent in untreated cells, were reversible upon cycling through heating and cooling scans, and attributable to lipid components of the membranes, possibly sphingolipids. These results suggest that, after treatment with methyl-beta-cyclodextrin, membranes may show thermotropic transitions, an unusual feature for cellular bilayers, which is likely to influence biological functions.

Masserini, M., Pitto, M., Raimondo, F., Cazzaniga, E., Sesana, M., Bellini, T. (2005). Methyl-beta-cyclodextrin treatment affects the thermotropic behaviour of membranes and detergent-resistant membrane fractions of cultured A431 cells. BIOLOGICAL & PHARMACEUTICAL BULLETIN, 28(12), 2185-2188 [10.1248/bpb.28.2185].

Methyl-beta-cyclodextrin treatment affects the thermotropic behaviour of membranes and detergent-resistant membrane fractions of cultured A431 cells

MASSERINI, MASSIMO ERNESTO;PITTO, MARINA;RAIMONDO, FRANCESCA;CAZZANIGA, EMANUELA;SESANA, MARIA SILVIA;BELLINI, TIZIANO
2005

Abstract

Membranes and detergent-resistant membrane fractions isolated from human epidermoid carcinoma A431 cells after treatment with methyl-beta-cyclodextrin, a compound commonly used in pharmaceutical applications and in manipulation of membrane cholesterol content, display thermotropic transitions at about 15 degrees C and above 37 degrees C, respectively, when analyzed by differential scanning calorimetry. The transitions, absent in untreated cells, were reversible upon cycling through heating and cooling scans, and attributable to lipid components of the membranes, possibly sphingolipids. These results suggest that, after treatment with methyl-beta-cyclodextrin, membranes may show thermotropic transitions, an unusual feature for cellular bilayers, which is likely to influence biological functions.
Articolo in rivista - Articolo scientifico
Cell Line, Tumor; Calorimetry, Differential Scanning; Temperature; beta-Cyclodextrins; Cell Fractionation; Membrane Lipids; Detergents; Phase Transition; Carcinoma, Squamous Cell; Humans; Cell Membrane
English
2185
2188
Masserini, M., Pitto, M., Raimondo, F., Cazzaniga, E., Sesana, M., Bellini, T. (2005). Methyl-beta-cyclodextrin treatment affects the thermotropic behaviour of membranes and detergent-resistant membrane fractions of cultured A431 cells. BIOLOGICAL & PHARMACEUTICAL BULLETIN, 28(12), 2185-2188 [10.1248/bpb.28.2185].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/13861
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