Caveolin-1 and flotillin-1 belong to plasma membrane microdomains. They are characterized by peculiar lipid and protein composition and are involved in fundamental cellular events such as: signal transduction, cell adhesion, lipid/protein sorting, and human cancer. We addressed caveolin-1 and flotillin-1 expression in 30 human renal cell carcinoma (RCC) and adjacent normal kidney (ANK) samples by SDS-PAGE and immunoblotting with specific antibodies. Significant caveolin-1 and flotillin-1 over-expression was found in RCC tissues compared to ANK, and was confirmed by immunohistochemistry. Caveolin-1 and flotillin-1 protein levels were found by 1-D, 2-DE, and MS to be increased also in RCC microdomain-enriched subcellular fractions purified from paired RCC and ANK samples.
Raimondo, F., Ticozzi Valerio, D., Magni, F., Perego, R., Bianchi, C., Sarto, C., et al. (2008). Caveolin-1 and Flotillin-1 Differential Expression in Clinical Samples of Renal Cell Carcinoma. THE OPEN PROTEOMICS JOURNAL, 1, 87-98 [10.2174/1875039700801010079].
Caveolin-1 and Flotillin-1 Differential Expression in Clinical Samples of Renal Cell Carcinoma
RAIMONDO, FRANCESCA;MAGNI, FULVIO;PEREGO, ROBERTO;BIANCHI, CRISTINA;KIENLE, MARZIA DONATELLA;BRAMBILLA, PAOLO;PITTO, MARINA
2008
Abstract
Caveolin-1 and flotillin-1 belong to plasma membrane microdomains. They are characterized by peculiar lipid and protein composition and are involved in fundamental cellular events such as: signal transduction, cell adhesion, lipid/protein sorting, and human cancer. We addressed caveolin-1 and flotillin-1 expression in 30 human renal cell carcinoma (RCC) and adjacent normal kidney (ANK) samples by SDS-PAGE and immunoblotting with specific antibodies. Significant caveolin-1 and flotillin-1 over-expression was found in RCC tissues compared to ANK, and was confirmed by immunohistochemistry. Caveolin-1 and flotillin-1 protein levels were found by 1-D, 2-DE, and MS to be increased also in RCC microdomain-enriched subcellular fractions purified from paired RCC and ANK samples.
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