Novel expression systems for the development of whole-cell biocatalysts were generated. Their novelty consists both in the host, Pseudomonas putida, and in the ability to auto-induce the expression of genes of interest at the exhaustion of the carbon source used for the biomass growth. The auto-induction relies on new expression vectors developed in this study and based on the activator TouR from Pseudomonas sp. OX1, which was shown to mediate the activation of target promoters in an effector-independent growth-phase-dependent manner when the carbon source is exhausted at the onset of the stationary phase. We validated the suitability of these expression systems through the production of (S)-styrene oxide by the styrene monooxygenase from Pseudomonas fluorescens ST. The yields of epoxides produced by these biocatalysts in flask experiments showed to be as efficient as those currently available based on inducible Escherichia coli systems. In addition, a larger scale of biomass production showed no reduction of biocatalysis efficiency. Therefore, the systems developed in this study constitute a valid alternative to current expression systems to use in bioconversion processes

DI GENNARO, P., Ferrara, S., Bestetti, G., Sello, G., Solera, D., Galli, E., et al. (2008). Novel auto-inducing expression systems for the development of whole-cell biocatalysts. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 79(4), 617-625 [10.1007/s00253-008-1460-z].

Novel auto-inducing expression systems for the development of whole-cell biocatalysts

DI GENNARO, PATRIZIA;BESTETTI, GIUSEPPINA;
2008

Abstract

Novel expression systems for the development of whole-cell biocatalysts were generated. Their novelty consists both in the host, Pseudomonas putida, and in the ability to auto-induce the expression of genes of interest at the exhaustion of the carbon source used for the biomass growth. The auto-induction relies on new expression vectors developed in this study and based on the activator TouR from Pseudomonas sp. OX1, which was shown to mediate the activation of target promoters in an effector-independent growth-phase-dependent manner when the carbon source is exhausted at the onset of the stationary phase. We validated the suitability of these expression systems through the production of (S)-styrene oxide by the styrene monooxygenase from Pseudomonas fluorescens ST. The yields of epoxides produced by these biocatalysts in flask experiments showed to be as efficient as those currently available based on inducible Escherichia coli systems. In addition, a larger scale of biomass production showed no reduction of biocatalysis efficiency. Therefore, the systems developed in this study constitute a valid alternative to current expression systems to use in bioconversion processes
Articolo in rivista - Articolo scientifico
Bacterial Proteins/genetics, Catalysis
English
2008
79
4
617
625
none
DI GENNARO, P., Ferrara, S., Bestetti, G., Sello, G., Solera, D., Galli, E., et al. (2008). Novel auto-inducing expression systems for the development of whole-cell biocatalysts. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 79(4), 617-625 [10.1007/s00253-008-1460-z].
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/13840
Citazioni
  • Scopus 6
  • ???jsp.display-item.citation.isi??? 5
Social impact