Renal cell carcinoma (RCC) is one of the major causes of cancer death and is radio- and chemoresistant. Urine of 29 healthy subjects and 39 clear cell RCC patients were analyzed using the ClinProt technique to search for possible biomarkers for early RCC diagnosis. A cluster of three signals (marker A= at m/z 1827 ± 8 Da, marker B = 1914 ± 8 Da and marker C = 1968 ± 8 Da) was able to discriminate patients from controls. A receiver operating characteristic curve analysis showed values of area under the curve (AUC) higher than 0.9 for marker A and B, corresponding to a sensitivity of 85-90% and a specificity of' 90%, while marker C gave a lower AUC (0.84) corresponding to sensitivity of 70% and specificity of 100%. The combination of three markers lead to an improvement in diagnostic efficacy, with specificity and sensitivity of 100% and 95%, respectively, in the training test and of 100% and of 85% in the test experiment. The efficacy of this cluster of signals to distinguish RCC patients grouped by tumor stage showed a sensibility of 100% for patients at the primary tumor 1 stage. One ofthe signals present in the cluster was identified as a fragment of Tamm-Horsfall protein. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Bosso, N., Chinello, C., Picozzi, S., Gianazza, E., Mainini, V., Galbusera, C., et al. (2008). Human urine biomarkers of renal cell carcinoma evaluated by ClinProt. PROTEOMICS. CLINICAL APPLICATIONS, 2(7-8), 1036-1046 [10.1002/prca.200780139].
|Citazione:||Bosso, N., Chinello, C., Picozzi, S., Gianazza, E., Mainini, V., Galbusera, C., et al. (2008). Human urine biomarkers of renal cell carcinoma evaluated by ClinProt. PROTEOMICS. CLINICAL APPLICATIONS, 2(7-8), 1036-1046 [10.1002/prca.200780139].|
|Tipo:||Articolo in rivista - Articolo scientifico|
|Carattere della pubblicazione:||Scientifica|
|Titolo:||Human urine biomarkers of renal cell carcinoma evaluated by ClinProt|
|Autori:||Bosso, N; Chinello, C; Picozzi, S; Gianazza, E; Mainini, V; Galbusera, C; Raimondo, F; Perego, R; Casellato, S; Rocco, F; Ferrero, S; Bosari, S; Mocarelli, P; Kienle, MD; Magni, F|
|Data di pubblicazione:||2008|
|Rivista:||PROTEOMICS. CLINICAL APPLICATIONS|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1002/prca.200780139|
|Appare nelle tipologie:||01 - Articolo su rivista|