Aquaporin (AQP)1 belongs to a ubiquitous family of water channel proteins characterized by sequence similarity and the presence of two NPA (Asp-Pro-Ala) motifs existing in almost all organs and tissues. Currently, 13 human AQPs are known and they are divided into two subgroups according to their ability to transport only water molecules, such as AQP1, or also glycerol and other small solutes. The genomic, structural and functional aspects of AQP1 are briefly described. An in-depth discussion is devoted to proteomic approaches that are useful for identifying and characterizing AQP1, mainly through electrophoretic techniques combined with different extraction procedures followed by mass spectrometry analysis. Moreover, the relevance of AQP1 in human diseases is also explained. Its role in human tumors and, in particular, those of the kidney (e.g., clear cell renal carcinoma) is discussed.

Magni, F., Chinello, C., Raimondo, F., Mocarelli, P., Kienle, M., Pitto, M. (2008). AQP1 expression analysis in human diseases: Implications for proteomic characterization. EXPERT REVIEW OF PROTEOMICS, 5(1), 29-43 [10.1586/14789450.5.1.29].

AQP1 expression analysis in human diseases: Implications for proteomic characterization

MAGNI, FULVIO;CHINELLO, CLIZIA;RAIMONDO, FRANCESCA;KIENLE, MARZIA DONATELLA;PITTO, MARINA
2008

Abstract

Aquaporin (AQP)1 belongs to a ubiquitous family of water channel proteins characterized by sequence similarity and the presence of two NPA (Asp-Pro-Ala) motifs existing in almost all organs and tissues. Currently, 13 human AQPs are known and they are divided into two subgroups according to their ability to transport only water molecules, such as AQP1, or also glycerol and other small solutes. The genomic, structural and functional aspects of AQP1 are briefly described. An in-depth discussion is devoted to proteomic approaches that are useful for identifying and characterizing AQP1, mainly through electrophoretic techniques combined with different extraction procedures followed by mass spectrometry analysis. Moreover, the relevance of AQP1 in human diseases is also explained. Its role in human tumors and, in particular, those of the kidney (e.g., clear cell renal carcinoma) is discussed.
Articolo in rivista - Articolo scientifico
Glycosylation; Proteomics; Neoplasm Proteins; Protein Conformation; Kidney Neoplasms; Neoplasms; Electrophoresis; Protein Processing, Post-Translational; Animals; Aquaporin 1; Digestive System; Humans; Models, Molecular; Water; Kidney Diseases; Kidney Cortex; Molecular Sequence Data; Mass Spectrometry; Brain; Amino Acid Sequence; Mammals; Carcinoma, Renal Cell
English
29
43
15
Magni, F., Chinello, C., Raimondo, F., Mocarelli, P., Kienle, M., Pitto, M. (2008). AQP1 expression analysis in human diseases: Implications for proteomic characterization. EXPERT REVIEW OF PROTEOMICS, 5(1), 29-43 [10.1586/14789450.5.1.29].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/13779
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