Direct cell reprogramming enables direct conversion of fibroblasts into functional neurons and oligodendrocytes using a minimal set of cell-lineage-specific transcription factors. This approach is rapid and simple, generating the cell types of interest in one step. However, it remains unknown whether this technology can be applied to convert fibroblasts into astrocytes, the third neural lineage. Astrocytes play crucial roles in neuronal homeostasis, and their dysfunctions contribute to the origin and progression of multiple human diseases. Herein, we carried out a screening using several transcription factors involved in defining the astroglial cell fate and identified NFIA, NFIB, and SOX9 to be sufficient to convert with high efficiency embryonic and postnatal mouse fibroblasts into astrocytes (iAstrocytes). We proved both by gene-expression profiling and functional tests that iAstrocytes are comparable to native brain astrocytes. This protocol can be then employed to generate functional iAstrocytes for a wide range of experimental applications.

Caiazzo, M., Giannelli, S., Valente, P., Lignani, G., Carissimo, A., Sessa, A., et al. (2015). Direct conversion of fibroblasts into functional astrocytes by defined transcription factors. STEM CELL REPORTS, 4(1), 25-36 [10.1016/j.stemcr.2014.12.002].

Direct conversion of fibroblasts into functional astrocytes by defined transcription factors

MASSIMINO, LUCA;
2015

Abstract

Direct cell reprogramming enables direct conversion of fibroblasts into functional neurons and oligodendrocytes using a minimal set of cell-lineage-specific transcription factors. This approach is rapid and simple, generating the cell types of interest in one step. However, it remains unknown whether this technology can be applied to convert fibroblasts into astrocytes, the third neural lineage. Astrocytes play crucial roles in neuronal homeostasis, and their dysfunctions contribute to the origin and progression of multiple human diseases. Herein, we carried out a screening using several transcription factors involved in defining the astroglial cell fate and identified NFIA, NFIB, and SOX9 to be sufficient to convert with high efficiency embryonic and postnatal mouse fibroblasts into astrocytes (iAstrocytes). We proved both by gene-expression profiling and functional tests that iAstrocytes are comparable to native brain astrocytes. This protocol can be then employed to generate functional iAstrocytes for a wide range of experimental applications.
Articolo in rivista - Articolo scientifico
Animals; Astrocytes; Biomarkers; Cell Transdifferentiation; Cells, Cultured; Cellular Reprogramming; Cluster Analysis; Cytokines; Fibroblasts; Gene Expression; Gene Expression Profiling; Humans; Membrane Potentials; Mice; Phenotype; Transcription Factors; Biochemistry; Cell Biology; Developmental Biology; Genetics
English
2015
4
1
25
36
reserved
Caiazzo, M., Giannelli, S., Valente, P., Lignani, G., Carissimo, A., Sessa, A., et al. (2015). Direct conversion of fibroblasts into functional astrocytes by defined transcription factors. STEM CELL REPORTS, 4(1), 25-36 [10.1016/j.stemcr.2014.12.002].
File in questo prodotto:
File Dimensione Formato  
Caiazzo et al. - 2015 - Direct Conversion of Fibroblasts into Functional Astrocytes by Defined Transcription Factors.pdf

Solo gestori archivio

Dimensione 2.34 MB
Formato Adobe PDF
2.34 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/137146
Citazioni
  • Scopus 166
  • ???jsp.display-item.citation.isi??? 152
Social impact