During cell division the main goal of the cell is to produce two daughter cells with the same genome as the mother, i.e., maintain its genetic stability. Since this issue is essential to preserve the cell ability to proliferate properly, all eukaryotic cells have developed several pathways, called mitotic checkpoints, that regulate mitotic entry, progression, and exit in response to different cellular signals. Given the evolutive conservation of mechanisms and proteins involved in the cell cycle control from yeast to humans, the budding yeast S. cerevisiae has been very helpful to gain insight in these complex regulations. Here, we describe how the checkpoint can be activated and which cellular phenotypes can be used as markers of checkpoint activation.

Fraschini, R. (2017). Analysis of the functionality of the mitotic checkpoints. In F. Monje-Casas, E. Queralt (a cura di), The Mitotic Exit Network - Methods and Protocols (pp. 217-228). Humana Press Inc. [10.1007/978-1-4939-6502-1_16].

Analysis of the functionality of the mitotic checkpoints

FRASCHINI, ROBERTA
Primo
2017

Abstract

During cell division the main goal of the cell is to produce two daughter cells with the same genome as the mother, i.e., maintain its genetic stability. Since this issue is essential to preserve the cell ability to proliferate properly, all eukaryotic cells have developed several pathways, called mitotic checkpoints, that regulate mitotic entry, progression, and exit in response to different cellular signals. Given the evolutive conservation of mechanisms and proteins involved in the cell cycle control from yeast to humans, the budding yeast S. cerevisiae has been very helpful to gain insight in these complex regulations. Here, we describe how the checkpoint can be activated and which cellular phenotypes can be used as markers of checkpoint activation.
Capitolo o saggio
Cell cycle; Cellular morphology; DNA content; MEN; Mitotic spindle; Nuclear positioning
English
The Mitotic Exit Network - Methods and Protocols
978-1-4939-6500-7
Fraschini, R. (2017). Analysis of the functionality of the mitotic checkpoints. In F. Monje-Casas, E. Queralt (a cura di), The Mitotic Exit Network - Methods and Protocols (pp. 217-228). Humana Press Inc. [10.1007/978-1-4939-6502-1_16].
Fraschini, R
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/135608
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