Pollen aeroallergens are present in atmospheric particulate matter (PM) where they can be found in coarse biological particles such as pollen grains (aerodynamic diameter dae > 10 μm), as well as fragments in the finest respirable particles (PM2.5; dae < 2.5 μm). Nitration of tyrosine residues in pollen allergenic proteins can occur in polluted air, and inhalation and deposition of these nitrated proteins in the human respiratory tract may lead to adverse health effects by enhancing the allergic response in population. Previous studies investigated protein nitration by atmospheric gaseous pollutants such as nitrogen dioxide and ozone. In this work we report, for the first time, a study on protein nitration by nitrate ion in aqueous solution, at nitrate concentrations and pH conditions simulating those occurring in the atmospheric aerosol liquid water phase. Experiments have been carried out on the Bovine serum albumin (BSA) protein and the recombinant Phleum pratense allergen (Phl p 2) both in the dark and under UV-A irradiation (range 4–90 W m− 2) to take into account thermal and/or photochemical nitration processes. For the latter protein, modifications in the allergic response after treatment with nitrate solutions have been evaluated by immunoblot analyses using sera from grass-allergic patients. Experimental results in bulk solutions showed that protein nitration in the dark occurs only in dilute nitrate solutions and under very acidic conditions (pH < 3 for BSA; pH < 2.2 for Phl p 2), while nitration is always observed (at pH 0.5–5) under UV-A irradiation, both in dilute and concentrated nitrate solutions, being significantly enhanced at the lowest pH values. In some cases, protein nitration resulted in an increase of the allergic response.

Ghiani, A., Bruschi, M., Citterio, S., Bolzacchini, E., Ferrero, L., Sangiorgi, G., et al. (2016). Nitration of pollen aeroallergens by nitrate ion in conditions simulating the liquid water phase of atmospheric particles. SCIENCE OF THE TOTAL ENVIRONMENT, 573, 1589-1597 [10.1016/j.scitotenv.2016.09.041].

Nitration of pollen aeroallergens by nitrate ion in conditions simulating the liquid water phase of atmospheric particles

GHIANI, ALESSANDRA
Primo
;
BRUSCHI, MAURIZIO
Secondo
;
CITTERIO, SANDRA;BOLZACCHINI, EZIO;FERRERO, LUCA;SANGIORGI, GIORGIA MAURA LUISA;PERRONE, MARIA GRAZIA
Ultimo
2016

Abstract

Pollen aeroallergens are present in atmospheric particulate matter (PM) where they can be found in coarse biological particles such as pollen grains (aerodynamic diameter dae > 10 μm), as well as fragments in the finest respirable particles (PM2.5; dae < 2.5 μm). Nitration of tyrosine residues in pollen allergenic proteins can occur in polluted air, and inhalation and deposition of these nitrated proteins in the human respiratory tract may lead to adverse health effects by enhancing the allergic response in population. Previous studies investigated protein nitration by atmospheric gaseous pollutants such as nitrogen dioxide and ozone. In this work we report, for the first time, a study on protein nitration by nitrate ion in aqueous solution, at nitrate concentrations and pH conditions simulating those occurring in the atmospheric aerosol liquid water phase. Experiments have been carried out on the Bovine serum albumin (BSA) protein and the recombinant Phleum pratense allergen (Phl p 2) both in the dark and under UV-A irradiation (range 4–90 W m− 2) to take into account thermal and/or photochemical nitration processes. For the latter protein, modifications in the allergic response after treatment with nitrate solutions have been evaluated by immunoblot analyses using sera from grass-allergic patients. Experimental results in bulk solutions showed that protein nitration in the dark occurs only in dilute nitrate solutions and under very acidic conditions (pH < 3 for BSA; pH < 2.2 for Phl p 2), while nitration is always observed (at pH 0.5–5) under UV-A irradiation, both in dilute and concentrated nitrate solutions, being significantly enhanced at the lowest pH values. In some cases, protein nitration resulted in an increase of the allergic response.
Articolo in rivista - Articolo scientifico
Acidic aerosol; Allergenicity; DRH; Slot Blot; Tyrosine nitration; UV-A irradiation;
Acidic aerosol; Allergenicity; DRH; Slot Blot; Tyrosine nitration; UV-A irradiation
English
1589
1597
9
Ghiani, A., Bruschi, M., Citterio, S., Bolzacchini, E., Ferrero, L., Sangiorgi, G., et al. (2016). Nitration of pollen aeroallergens by nitrate ion in conditions simulating the liquid water phase of atmospheric particles. SCIENCE OF THE TOTAL ENVIRONMENT, 573, 1589-1597 [10.1016/j.scitotenv.2016.09.041].
Ghiani, A; Bruschi, M; Citterio, S; Bolzacchini, E; Ferrero, L; Sangiorgi, G; Asero, R; Perrone, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/135228
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