A growing body of evidence links the analysis of the KIR genotype and the presence of their HLA-B and -C ligands to a wide repertoire of human diseases.We noticed that, using a panel of 184 Caucasoid donors, a limited number of HLA alleles were incorrectly supratyped by previously described pyrosequence-based assays. Here we describe a simple implementation of the reported methods that corrects all the discrepancies found with HLA-B and -C molecular typing and allows establishing a quick and high-throughput method for the determination of HLA-Bw4 I80, Bw4T80, Bw6 and HLA-C1 or -C2 supratype.
Biassoni, R., Malnati, M., Vanni, I., Raso, A., Sironi, F., Broccolo, F., et al. (2015). An improved method for HLA-B and -C supratyping. JOURNAL OF IMMUNOLOGICAL METHODS, 426, 29-34.
Citazione: | Biassoni, R., Malnati, M., Vanni, I., Raso, A., Sironi, F., Broccolo, F., et al. (2015). An improved method for HLA-B and -C supratyping. JOURNAL OF IMMUNOLOGICAL METHODS, 426, 29-34. |
Tipo: | Articolo in rivista - Articolo scientifico |
Carattere della pubblicazione: | Scientifica |
Presenza di un coautore afferente ad Istituzioni straniere: | No |
Titolo: | An improved method for HLA-B and -C supratyping |
Autori: | Biassoni, R; Malnati, M; Vanni, I; Raso, A; Sironi, F; Broccolo, F; Garbarino, L; Mazzocco, M; Montera, M; Larghero, P; Di Marco, E; Ugolotti, E |
Autori: | UGOLOTTI, EMILIA (Ultimo) |
Data di pubblicazione: | 2015 |
Lingua: | English |
Rivista: | JOURNAL OF IMMUNOLOGICAL METHODS |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.jim.2015.07.008 |
Appare nelle tipologie: | 01 - Articolo su rivista |