A growing body of evidence links the analysis of the KIR genotype and the presence of their HLA-B and -C ligands to a wide repertoire of human diseases.We noticed that, using a panel of 184 Caucasoid donors, a limited number of HLA alleles were incorrectly supratyped by previously described pyrosequence-based assays. Here we describe a simple implementation of the reported methods that corrects all the discrepancies found with HLA-B and -C molecular typing and allows establishing a quick and high-throughput method for the determination of HLA-Bw4 I80, Bw4T80, Bw6 and HLA-C1 or -C2 supratype.
Biassoni, R., Malnati, M., Vanni, I., Raso, A., Sironi, F., Broccolo, F., et al. (2015). An improved method for HLA-B and -C supratyping. JOURNAL OF IMMUNOLOGICAL METHODS, 426, 29-34 [10.1016/j.jim.2015.07.008].
An improved method for HLA-B and -C supratyping
SIRONI, FRANCESCA;BROCCOLO, FRANCESCO;UGOLOTTI, EMILIAUltimo
2015
Abstract
A growing body of evidence links the analysis of the KIR genotype and the presence of their HLA-B and -C ligands to a wide repertoire of human diseases.We noticed that, using a panel of 184 Caucasoid donors, a limited number of HLA alleles were incorrectly supratyped by previously described pyrosequence-based assays. Here we describe a simple implementation of the reported methods that corrects all the discrepancies found with HLA-B and -C molecular typing and allows establishing a quick and high-throughput method for the determination of HLA-Bw4 I80, Bw4T80, Bw6 and HLA-C1 or -C2 supratype.
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