The investigated contaminated site is situated in the proximity of a former crude oil refinery. Contamination took place in the 1970s, following the spilling of huge amount of diesel. GC/MS analyses demonstrate that the major components of nonaqueous liquid phase contaminant mixture are branched alkanes and alkyl-substituted naphthalenes. In the site, bioslurping technology was under experimental investigation. The aim of this work was: (1) to evaluate whether the weathered diesel hydrocarbons present as residual contamination can be further degraded by stimulation of indigenous microorganisms; (2) to monitor the effect of the bioslurping on the hydrocarbondegrading bacterial community; and (3) to characterize the weathered diesel-degrading bacterial community. Enumeration of hydrocarbon-degrading bacteria and respirometric tests were carried out during bioslurping treatment. An increase in number and metabolic activity of degrading bacteria was demonstrated. Hydrocarbon-degrading bacteria enriched from capillary fringe and from the NAPL samples were isolated and characterized by 16S ribosomal RNA gene analysis. The majority of the isolates were assigned to the Actinomycetales or to the Rhizobiales orders. A new Gordonia strain (M22) was selected and its degradation capabilities were evaluated. Gordonia sp. M22 strain was capable of degrading the major components of the contaminant mixture.
Caredda, P., La Colla, P., Tamburini, E., Viola, A., Suardi, A., Franzetti, A., et al. (2007). Laboratory Tests and Bioremediation of a Chronically Diesel-Contaminated Site. In Proceedings of The 9th International symposium on In-Situ and On-Site bioremediation. Batelle Press.
Laboratory Tests and Bioremediation of a Chronically Diesel-Contaminated Site
FRANZETTI, ANDREA;BESTETTI, GIUSEPPINA
2007
Abstract
The investigated contaminated site is situated in the proximity of a former crude oil refinery. Contamination took place in the 1970s, following the spilling of huge amount of diesel. GC/MS analyses demonstrate that the major components of nonaqueous liquid phase contaminant mixture are branched alkanes and alkyl-substituted naphthalenes. In the site, bioslurping technology was under experimental investigation. The aim of this work was: (1) to evaluate whether the weathered diesel hydrocarbons present as residual contamination can be further degraded by stimulation of indigenous microorganisms; (2) to monitor the effect of the bioslurping on the hydrocarbondegrading bacterial community; and (3) to characterize the weathered diesel-degrading bacterial community. Enumeration of hydrocarbon-degrading bacteria and respirometric tests were carried out during bioslurping treatment. An increase in number and metabolic activity of degrading bacteria was demonstrated. Hydrocarbon-degrading bacteria enriched from capillary fringe and from the NAPL samples were isolated and characterized by 16S ribosomal RNA gene analysis. The majority of the isolates were assigned to the Actinomycetales or to the Rhizobiales orders. A new Gordonia strain (M22) was selected and its degradation capabilities were evaluated. Gordonia sp. M22 strain was capable of degrading the major components of the contaminant mixture.
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