Dendritic cells (DCs) have an important role in the activation of NK cells that exert direct antitumor and antimicrobial effects and can influence the development of adaptive T cell responses. DCs acquire NK cell stimulatory capacity after exposure to various stimuli. In this study we investigated the nature of the stimuli that confer to DCs the NK cell-activating capacity. After exposure of DCs to TLR-dependent and -independent microbial stimuli and to nonmicrobial stimuli, we evaluated the ability of activated DCs to elicit IFN-gamma production from NK cells in vitro and to promote NK cell activation in vivo. We show in this study that only TLR-dependent microbial stimuli typically associated with Th1 responses confer to DCs the ability to activate NK cells, whereas stimuli associated with Th2 responses do not have this property.

Zanoni, I., Foti, M., Castagnoli, P., Granucci, F. (2005). TLR-dependent activation stimuli associated with Th1 responses confer NK cell stimulatory capacity to mouse dendritic cells. JOURNAL OF IMMUNOLOGY, 175(1), 286-292 [10.4049/jimmunol.175.1.286].

TLR-dependent activation stimuli associated with Th1 responses confer NK cell stimulatory capacity to mouse dendritic cells

ZANONI, IVAN;FOTI, MARIA;CASTAGNOLI, PAOLA;GRANUCCI, FRANCESCA
2005

Abstract

Dendritic cells (DCs) have an important role in the activation of NK cells that exert direct antitumor and antimicrobial effects and can influence the development of adaptive T cell responses. DCs acquire NK cell stimulatory capacity after exposure to various stimuli. In this study we investigated the nature of the stimuli that confer to DCs the NK cell-activating capacity. After exposure of DCs to TLR-dependent and -independent microbial stimuli and to nonmicrobial stimuli, we evaluated the ability of activated DCs to elicit IFN-gamma production from NK cells in vitro and to promote NK cell activation in vivo. We show in this study that only TLR-dependent microbial stimuli typically associated with Th1 responses confer to DCs the ability to activate NK cells, whereas stimuli associated with Th2 responses do not have this property.
Articolo in rivista - Articolo scientifico
Membrane Glycoproteins; Coculture Techniques; Th1 Cells; Lymphocyte Activation; Cysteine; Lipoproteins; Animals; Lipopolysaccharides; Receptors, Cell Surface; Killer Cells, Natural; Interferon-gamma; Dendritic Cells; Mycobacterium bovis; Mice; Toll-Like Receptors; Leishmania mexicana; Mice, Inbred C57BL; CpG Islands; Mice, Inbred BALB C
English
2005
175
1
286
292
reserved
Zanoni, I., Foti, M., Castagnoli, P., Granucci, F. (2005). TLR-dependent activation stimuli associated with Th1 responses confer NK cell stimulatory capacity to mouse dendritic cells. JOURNAL OF IMMUNOLOGY, 175(1), 286-292 [10.4049/jimmunol.175.1.286].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/13252
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