Junctional Adhesion Molecule-A (JAM-A) is a transmembrane adhesive protein expressed at endothelial junctions and in leukocytes. Here we report that JAM-A is required for the correct infiltration of polymorphonuclear leukocytes (PMN) into an inflamed peritoneum or in the heart upon ischemia-reperfusion injury. The defect was not observed in mice with an endothelium-restricted deficiency of the protein but was still detectable in mice transplanted with bone marrow from JAM-A(-/-) donors. Microscopic examination of mesenteric and heart microvasculature of JAM-A(-/-) mice showed high numbers of PMN adherent on the endothelium or entrapped between endothelial cells and the basement membrane. In vitro, in the absence of JAM-A, PMN adhered more efficiently to endothelial cells and basement membrane proteins, and their polarized movement was strongly reduced. This paper describes a nonredundant role of JAM-A in controlling PMN diapedesis through the vessel wall

Corada, M., Chimenti, S., Cera, M., Vinci, M., Salio, M., Fiordaliso, F., et al. (2005). Junctional adhesion molecule-A-deficient polyrnorphonuclear cells show reduced diapedesis in peritonitis and heart ischemia-reperfusion injury. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 102(30), 10634-10639 [10.1073/pnas.0500147102].

Junctional adhesion molecule-A-deficient polyrnorphonuclear cells show reduced diapedesis in peritonitis and heart ischemia-reperfusion injury

VILLA, ANTONELLO;BOSSI, MARIO;
2005

Abstract

Junctional Adhesion Molecule-A (JAM-A) is a transmembrane adhesive protein expressed at endothelial junctions and in leukocytes. Here we report that JAM-A is required for the correct infiltration of polymorphonuclear leukocytes (PMN) into an inflamed peritoneum or in the heart upon ischemia-reperfusion injury. The defect was not observed in mice with an endothelium-restricted deficiency of the protein but was still detectable in mice transplanted with bone marrow from JAM-A(-/-) donors. Microscopic examination of mesenteric and heart microvasculature of JAM-A(-/-) mice showed high numbers of PMN adherent on the endothelium or entrapped between endothelial cells and the basement membrane. In vitro, in the absence of JAM-A, PMN adhered more efficiently to endothelial cells and basement membrane proteins, and their polarized movement was strongly reduced. This paper describes a nonredundant role of JAM-A in controlling PMN diapedesis through the vessel wall
Articolo in rivista - Articolo scientifico
endothelial cell junctions; leukocyte transmigration; myocardial infarction; polymorphonuclear leukocytes
English
2005
102
30
10634
10639
none
Corada, M., Chimenti, S., Cera, M., Vinci, M., Salio, M., Fiordaliso, F., et al. (2005). Junctional adhesion molecule-A-deficient polyrnorphonuclear cells show reduced diapedesis in peritonitis and heart ischemia-reperfusion injury. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 102(30), 10634-10639 [10.1073/pnas.0500147102].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/13182
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