We verified the feasibility of plasma bound method for detecting renal cell carcinoma (RCC) combining the study of plasma DNA concentration and microsatellite alterations (LOH). Plasma DNA concentration was evaluated with real-time PCR in 54 patients with renal neoplasm before surgery and in 20 of these patients during a 26-64 month follow-up. Microsatellite study was performed on tumour tissue DNA of 33 RCC clear cell (RCCcc) and on plasma DNA of 14 RCCcc patients during preoperative and/or follow-up period. Patients had a significantly high (26.4+/-48.3 ng/ml versus controls 3.2+/-1.5 ng/ml; p=0.003) preoperative plasma DNA concentration that decreased after nephrectomy. During follow-up, plasma DNA increased in 12 patients without evidence of neoplasia; 3 patients successively relapsed. Tumour tissue DNA of 25 RCCcc patients (75.8%) displayed microsatellite LOH. Preoperative plasma DNA of 9 patients harboured LOH in 5 cases (55.6%). Augmented plasma DNA of 7 patients displayed LOH in 3 cases (42.9%) at follow-up, and in 1 case preceded the recurrence of disease. Plasma DNA concentration combined with microsatellite LOH in plasma DNA may predict disease recurrence in RCC patients.

Perego, R., Corizzato, M., Brambilla, P., Ferrero, S., Bianchi, C., Fasoli, E., et al. (2008). Concentration and microsatellite status of plasma DNA for monitoring patients with renal carcinoma. EUROPEAN JOURNAL OF CANCER, 44(7), 1039-1047 [10.1016/j.ejca.2008.03.008].

Concentration and microsatellite status of plasma DNA for monitoring patients with renal carcinoma

PEREGO, ROBERTO;CORIZZATO, MATTEO;BRAMBILLA, PAOLA;BIANCHI, CRISTINA;TORSELLO, BARBARA ROSA;INVERNIZZI, LARA;BOMBELLI, SILVIA;ANGELONI, VALENTINA;PITTO, MARINA;MAGNI, FULVIO;MOCARELLI, PAOLO
2008

Abstract

We verified the feasibility of plasma bound method for detecting renal cell carcinoma (RCC) combining the study of plasma DNA concentration and microsatellite alterations (LOH). Plasma DNA concentration was evaluated with real-time PCR in 54 patients with renal neoplasm before surgery and in 20 of these patients during a 26-64 month follow-up. Microsatellite study was performed on tumour tissue DNA of 33 RCC clear cell (RCCcc) and on plasma DNA of 14 RCCcc patients during preoperative and/or follow-up period. Patients had a significantly high (26.4+/-48.3 ng/ml versus controls 3.2+/-1.5 ng/ml; p=0.003) preoperative plasma DNA concentration that decreased after nephrectomy. During follow-up, plasma DNA increased in 12 patients without evidence of neoplasia; 3 patients successively relapsed. Tumour tissue DNA of 25 RCCcc patients (75.8%) displayed microsatellite LOH. Preoperative plasma DNA of 9 patients harboured LOH in 5 cases (55.6%). Augmented plasma DNA of 7 patients displayed LOH in 3 cases (42.9%) at follow-up, and in 1 case preceded the recurrence of disease. Plasma DNA concentration combined with microsatellite LOH in plasma DNA may predict disease recurrence in RCC patients.
Articolo in rivista - Articolo scientifico
Male; Aged, 80 and over; Cell Proliferation; ROC Curve; Middle Aged; Loss of Heterozygosity; Chromosomes, Human, Pair 3; Microcirculation; Female; Feasibility Studies; Kidney Neoplasms; Humans; DNA, Neoplasm; Follow-Up Studies; Microsatellite Repeats; Aged; Adult; Carcinoma, Renal Cell; Case-Control Studies
English
1039
1047
9
Perego, R., Corizzato, M., Brambilla, P., Ferrero, S., Bianchi, C., Fasoli, E., et al. (2008). Concentration and microsatellite status of plasma DNA for monitoring patients with renal carcinoma. EUROPEAN JOURNAL OF CANCER, 44(7), 1039-1047 [10.1016/j.ejca.2008.03.008].
Perego, R; Corizzato, M; Brambilla, P; Ferrero, S; Bianchi, C; Fasoli, E; Signorini, S; Torsello, B; Invernizzi, L; Bombelli, S; Angeloni, V; Pitto, M; Battaglia, C; Proserpio, V; Magni, F; Galasso, G; Mocarelli, P
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/13069
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