Proteomics methodologies hold great promise in basic renal research and clinical nephrology. The classical approach for proteomic analysis couples two-dimensional gel electrophoresis (2-DE) with protein identification by mass spectrometry, to produce more global information regarding normal protein expression and alterations in different physiological and pathological states. In this report we have expanded the identification of proteins in the renal cortex, improving the previously published map to facilitate the study of different diseases affecting the human kidney. About 250 spots were analyzed by peptide mass fingerprinting, 89 proteins and 74 isoforms for some of them were identified and implemented in the normal human renal cortex 2-DE reference map. This more comprehensive view of the proteome of the human renal cortex could be of invaluable help to the differential proteomic display of urological diseases.

Magni, F., Sarto, C., Valsecchi, C., Casellato, S., Bogetto, S., Bosari, S., et al. (2005). Expanding the proteome two-dimensional gel electrophoresis reference map of human renal cortex by peptide mass fingerprinting. PROTEOMICS, 5(3), 816-825 [10.1002/pmic.200401077].

Expanding the proteome two-dimensional gel electrophoresis reference map of human renal cortex by peptide mass fingerprinting

MAGNI, FULVIO;PEREGO, ROBERTO;GALBUSERA, CARMEN;KIENLE, MARZIA DONATELLA
2005

Abstract

Proteomics methodologies hold great promise in basic renal research and clinical nephrology. The classical approach for proteomic analysis couples two-dimensional gel electrophoresis (2-DE) with protein identification by mass spectrometry, to produce more global information regarding normal protein expression and alterations in different physiological and pathological states. In this report we have expanded the identification of proteins in the renal cortex, improving the previously published map to facilitate the study of different diseases affecting the human kidney. About 250 spots were analyzed by peptide mass fingerprinting, 89 proteins and 74 isoforms for some of them were identified and implemented in the normal human renal cortex 2-DE reference map. This more comprehensive view of the proteome of the human renal cortex could be of invaluable help to the differential proteomic display of urological diseases.
No
Articolo in rivista - Articolo scientifico
Scientifica
Human renal cortex, Kidney master image, Mass spectrometry, Two-dimensional gel electrophoresis, Peptide mass fingerprinting
English
816
825
10
Magni, F., Sarto, C., Valsecchi, C., Casellato, S., Bogetto, S., Bosari, S., et al. (2005). Expanding the proteome two-dimensional gel electrophoresis reference map of human renal cortex by peptide mass fingerprinting. PROTEOMICS, 5(3), 816-825 [10.1002/pmic.200401077].
Magni, F; Sarto, C; Valsecchi, C; Casellato, S; Bogetto, S; Bosari, S; Di Fonzo, A; Perego, R; Corizzato, M; Doro, G; Galbusera, C; Rocco, F; Mocarelli, P; Kienle, M
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/13058
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