Mammalian metallocarboxypeptidases play key roles in major biological processes, such as digestive-protein degradation and specific proteolytic processing. A Sulfolobus solfataricus gene (cpsA) encoding a recently described zinc carboxypeptidase with an unusually broad substrate specificity was cloned, sequenced, and expressed in Escherichia coli. Despite the lack of overall sequence homology with known carboxypeptidases, seven homology blocks, including the Zn-coordinating and catalytic residues, were identified by multiple alignment with carboxypeptidases A, B, and T. S. solfataricus carboxypeptidase expressed in E. coli was found to be enzymatically active, and both its substrate specificity and thermostability were comparable to those of the purified S. solfataricus enzyme.

Colombo, S., Toietta, G., Zecca, L., Vanoni, M., Tortora, P. (1995). Molecular cloning, nucleotide sequence and expression of a carboxypeptidase-encoding gene from the archaebacterium Sulfolobus solfataricus. JOURNAL OF BACTERIOLOGY, 177(19), 5561-5566.

Molecular cloning, nucleotide sequence and expression of a carboxypeptidase-encoding gene from the archaebacterium Sulfolobus solfataricus

COLOMBO, SONIA;VANONI, MARCO ERCOLE;TORTORA, PAOLO
1995

Abstract

Mammalian metallocarboxypeptidases play key roles in major biological processes, such as digestive-protein degradation and specific proteolytic processing. A Sulfolobus solfataricus gene (cpsA) encoding a recently described zinc carboxypeptidase with an unusually broad substrate specificity was cloned, sequenced, and expressed in Escherichia coli. Despite the lack of overall sequence homology with known carboxypeptidases, seven homology blocks, including the Zn-coordinating and catalytic residues, were identified by multiple alignment with carboxypeptidases A, B, and T. S. solfataricus carboxypeptidase expressed in E. coli was found to be enzymatically active, and both its substrate specificity and thermostability were comparable to those of the purified S. solfataricus enzyme.
Articolo in rivista - Articolo scientifico
Archaebacteria, Thermostable enzyme, Sulfolobus solfataricus
English
5561
5566
Colombo, S., Toietta, G., Zecca, L., Vanoni, M., Tortora, P. (1995). Molecular cloning, nucleotide sequence and expression of a carboxypeptidase-encoding gene from the archaebacterium Sulfolobus solfataricus. JOURNAL OF BACTERIOLOGY, 177(19), 5561-5566.
Colombo, S; Toietta, G; Zecca, L; Vanoni, M; Tortora, P
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/12453
Citazioni
  • Scopus 33
  • ???jsp.display-item.citation.isi??? 29
Social impact