Diabetes mellitus accelerates atherosclerotic processes, and it is known that inflammation plays a key role in atherosclerosis. The aim of the present study was to evaluate in patients with Type 2 diabetes whether serum levels of CRP (C-reactive protein) are associated with cytokine production in whole blood. A total of 89 outpatients with Type 2 diabetes were enrolled, and blood pressure, body mass index, fasting blood glucose, glycated haemoglobin, cholesterol, triacylglycerols (triglycerides) and hs-CRP (high-sensitivity CRP) were measured. IL-6 (interleukin-6), IL-1β (interleukin-1β) and TNF-α (tumour necrosis factor-α) were measured before and after 24 h of incubation of whole blood with LPS (lipopolysaccharide) or saline. The basal values of IL-1β, IL-6 and TNF-α were low and were not significantly related to hs-CRP levels. A univariate analysis showed that the level of IL-1β and IL-6, obtained after 24 h of incubation of whole blood with LPS, increased significantly with increasing levels of hs-CRP and, after adjusting for potential confounders, IL-1β still remained statistically significant. In our sample of patients with Type 2 diabetes, there was no association between serum hs-CRP levels and basal levels of IL-6, IL-1β and TNF-α. Conversely, a significant association was observed between serum hs-CRP levels and IL-1β and IL-6 production after 24 h of incubation of whole blood with LPS. In conclusion, our data suggest that patients with Type 2 diabetes and high hs-CRP levels may have an enhanced reactivity in response to specific stimuli that produce different interleukins, with possible implications in inflammatory atherosclerotic processes. © 2007 The Biochemical Society.

Castoldi, G., Galimberti, S., Riva, C., Papagna, R., Querci, F., Casati, M., et al. (2007). Association between serum values of C-reactive protein and cytokine production in whole blood of patients with Type 2 diabetes. CLINICAL SCIENCE, 113(1-2), 103-108 [10.1042/CS20060338].

Association between serum values of C-reactive protein and cytokine production in whole blood of patients with Type 2 diabetes

Castoldi, G;GALIMBERTI, STEFANIA;CACCIANIGA, GIANLUIGI;FERRARESE, CARLO;BALDONI, MARCO GIOVANNI;STELLA, ANDREA;VALSECCHI, MARIA GRAZIA
2007

Abstract

Diabetes mellitus accelerates atherosclerotic processes, and it is known that inflammation plays a key role in atherosclerosis. The aim of the present study was to evaluate in patients with Type 2 diabetes whether serum levels of CRP (C-reactive protein) are associated with cytokine production in whole blood. A total of 89 outpatients with Type 2 diabetes were enrolled, and blood pressure, body mass index, fasting blood glucose, glycated haemoglobin, cholesterol, triacylglycerols (triglycerides) and hs-CRP (high-sensitivity CRP) were measured. IL-6 (interleukin-6), IL-1β (interleukin-1β) and TNF-α (tumour necrosis factor-α) were measured before and after 24 h of incubation of whole blood with LPS (lipopolysaccharide) or saline. The basal values of IL-1β, IL-6 and TNF-α were low and were not significantly related to hs-CRP levels. A univariate analysis showed that the level of IL-1β and IL-6, obtained after 24 h of incubation of whole blood with LPS, increased significantly with increasing levels of hs-CRP and, after adjusting for potential confounders, IL-1β still remained statistically significant. In our sample of patients with Type 2 diabetes, there was no association between serum hs-CRP levels and basal levels of IL-6, IL-1β and TNF-α. Conversely, a significant association was observed between serum hs-CRP levels and IL-1β and IL-6 production after 24 h of incubation of whole blood with LPS. In conclusion, our data suggest that patients with Type 2 diabetes and high hs-CRP levels may have an enhanced reactivity in response to specific stimuli that produce different interleukins, with possible implications in inflammatory atherosclerotic processes. © 2007 The Biochemical Society.
Articolo in rivista - Articolo scientifico
C-REACTIVE PROTEIN, CYTOKINE, DIABETES
English
2007
113
1-2
103
108
none
Castoldi, G., Galimberti, S., Riva, C., Papagna, R., Querci, F., Casati, M., et al. (2007). Association between serum values of C-reactive protein and cytokine production in whole blood of patients with Type 2 diabetes. CLINICAL SCIENCE, 113(1-2), 103-108 [10.1042/CS20060338].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/11922
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