UBP10 encodes a deubiquitinating enzyme of Saccharomyces cerevisiae. Its inactivation results in a complex phenotype characterized by a subpopulation of cells that exhibits the typical cellular markers of apoptosis. Here, we show that additional deletion of YCA1, coding for the yeast metacaspase, suppressed the ubp10 disruptant phenotype. Moreover, YCA1 overexpression, without any external stimulus, had a detrimental effect on growth and viability of ubp10 cells accompanied by an increase of apoptotic cells. This response was completely abrogated by ascorbic acid addition. We also observed that cells lacking UBP10 had an endogenous caspase activity, revealed by incubation in vivo with FITC-labeled VAD-fmk. All these results argue in favour of an involvement of the yeast metacaspase in the active cell death triggered by loss of UBP10 function. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Bettiga, M., Calzari, L., Orlandi, I., Alberghina, L., Vai, M. (2004). Involvement of the yeast metacaspase Yca1 in ubp10-programmed cell death. FEMS YEAST RESEARCH, 5(2), 141-147 [10.1016/j.femsyr.2004.07.005].

Involvement of the yeast metacaspase Yca1 in ubp10-programmed cell death

BETTIGA, MAURIZIO;ORLANDI, IVAN;ALBERGHINA, LILIA;VAI, MARINA
2004

Abstract

UBP10 encodes a deubiquitinating enzyme of Saccharomyces cerevisiae. Its inactivation results in a complex phenotype characterized by a subpopulation of cells that exhibits the typical cellular markers of apoptosis. Here, we show that additional deletion of YCA1, coding for the yeast metacaspase, suppressed the ubp10 disruptant phenotype. Moreover, YCA1 overexpression, without any external stimulus, had a detrimental effect on growth and viability of ubp10 cells accompanied by an increase of apoptotic cells. This response was completely abrogated by ascorbic acid addition. We also observed that cells lacking UBP10 had an endogenous caspase activity, revealed by incubation in vivo with FITC-labeled VAD-fmk. All these results argue in favour of an involvement of the yeast metacaspase in the active cell death triggered by loss of UBP10 function. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Articolo in rivista - Articolo scientifico
apoptosis, deubiquitinating enzyme, metacaspase,ascorbic acid
English
141
147
Bettiga, M., Calzari, L., Orlandi, I., Alberghina, L., Vai, M. (2004). Involvement of the yeast metacaspase Yca1 in ubp10-programmed cell death. FEMS YEAST RESEARCH, 5(2), 141-147 [10.1016/j.femsyr.2004.07.005].
Bettiga, M; Calzari, L; Orlandi, I; Alberghina, L; Vai, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/11842
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