Novel approaches for high contrast, deep tissue, in vivo fluorescence biomedical imaging are based on infrared-emitting nanoparticles working in the so-called second biological window (1,000–1,400 nm). This allows for the acquisition of high resolution, deep tissue images due to the partial transparency of tissues in this particular spectral range. In addition, the optical excitation with low energy (infrared) photons also leads to a drastic reduction in the contribution of autofluorescence to the in vivo image. Nevertheless, as is demonstrated here, working solely in this biological window does not ensure a complete removal of autofluorescence as the specimen’s diet shows a remarkable infrared fluorescence that extends up to 1,100 nm. In this work, we show how the 1,340 nm emission band of Nd3+ ions embedded in SrF2 nanoparticles can be used to produce autofluorescence free, high contrast in vivo fluorescence images. It is also demonstrated that the complete removal of the food-related infrared autofluorescence is imperative for the development of reliable biodistribution studies.

Villa, I., Vedda, A., Cantarelli, I., Pedroni, M., Piccinelli, F., Bettinelli, M., et al. (2015). 1.3 μm emitting SrF2:Nd3+ nanoparticles for high contrast in vivo imaging in the second biological window. NANO RESEARCH, 8(2), 649-665 [10.1007/s12274-014-0549-1].

1.3 μm emitting SrF2:Nd3+ nanoparticles for high contrast in vivo imaging in the second biological window

Villa, I;Vedda, A;
2015

Abstract

Novel approaches for high contrast, deep tissue, in vivo fluorescence biomedical imaging are based on infrared-emitting nanoparticles working in the so-called second biological window (1,000–1,400 nm). This allows for the acquisition of high resolution, deep tissue images due to the partial transparency of tissues in this particular spectral range. In addition, the optical excitation with low energy (infrared) photons also leads to a drastic reduction in the contribution of autofluorescence to the in vivo image. Nevertheless, as is demonstrated here, working solely in this biological window does not ensure a complete removal of autofluorescence as the specimen’s diet shows a remarkable infrared fluorescence that extends up to 1,100 nm. In this work, we show how the 1,340 nm emission band of Nd3+ ions embedded in SrF2 nanoparticles can be used to produce autofluorescence free, high contrast in vivo fluorescence images. It is also demonstrated that the complete removal of the food-related infrared autofluorescence is imperative for the development of reliable biodistribution studies.
Articolo in rivista - Articolo scientifico
Scientifica
fluorescence imaging; nanomedicine; rare earth doped nanoparticles;
fluorescence imaging; nanomedicine; rare earth doped nanoparticles; Infrared Up-Conversion; Ag2s Quantum Dots; Doped Laf3 Nanoparticles; Gold Nanoparticles; Carbon Nanotubes; Cancer-Therapy; Drug-Delivery; Fluorescence; Mice; Nd3+;
English
Villa, I., Vedda, A., Cantarelli, I., Pedroni, M., Piccinelli, F., Bettinelli, M., et al. (2015). 1.3 μm emitting SrF2:Nd3+ nanoparticles for high contrast in vivo imaging in the second biological window. NANO RESEARCH, 8(2), 649-665 [10.1007/s12274-014-0549-1].
Villa, I; Vedda, A; Cantarelli, I; Pedroni, M; Piccinelli, F; Bettinelli, M; Speghini, A; Quintanilla, M; Vetrone, F; Rocha, U; Jacinto, C; Carrasco, E; Rodríguez, F; Juarranz, Á; del Rosal, B; Ortgies, D; Gonzalez, P; Solé, J; García, D
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/111709
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