The expression of recombinant human growth hormone (h-GH) and human interferon-alpha-2b (IFN-alpha-2b) in E. coli leads to the formation of insoluble protein aggregates or inclusion bodies (IBs). The secondary structure of these IBs, their corresponding native forms and thermal aggregates were studied by Fourier Transform Infrared (FT-IR) spectroscopy and microspectroscopy. It was demonstrated that residual native-like structures were maintained within IBs at different extents depending on the level of expression, with possible implications in biotechnology. Furthermore, comparison between infrared spectra of thermal aggregates and IBs suggests new insights on the structure of protein aggregates.

Ami, D., Natalello, A., Taylor, G., Tonon, G., & Doglia, S. (2006). Structural analysis of protein inclusion bodies by Fourier transform infrared microspectroscopy. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1764(4), 793-799 [10.1016/j.bbapap.2005.12.005].

Structural analysis of protein inclusion bodies by Fourier transform infrared microspectroscopy

AMI, DILETTA;NATALELLO, ANTONINO;DOGLIA, SILVIA MARIA
2006

Abstract

The expression of recombinant human growth hormone (h-GH) and human interferon-alpha-2b (IFN-alpha-2b) in E. coli leads to the formation of insoluble protein aggregates or inclusion bodies (IBs). The secondary structure of these IBs, their corresponding native forms and thermal aggregates were studied by Fourier Transform Infrared (FT-IR) spectroscopy and microspectroscopy. It was demonstrated that residual native-like structures were maintained within IBs at different extents depending on the level of expression, with possible implications in biotechnology. Furthermore, comparison between infrared spectra of thermal aggregates and IBs suggests new insights on the structure of protein aggregates.
Articolo in rivista - Articolo scientifico
Scientifica
protein aggregation; inclusion bodies; heterologous protein production; FT-IR spectroscopy; human growth hormone; interferon alpha
English
793
799
Ami, D., Natalello, A., Taylor, G., Tonon, G., & Doglia, S. (2006). Structural analysis of protein inclusion bodies by Fourier transform infrared microspectroscopy. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1764(4), 793-799 [10.1016/j.bbapap.2005.12.005].
Ami, D; Natalello, A; Taylor, G; Tonon, G; Doglia, S
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/1076
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