Prep1 is known to interact in vivo with Pbx1 to regulate development and organogenesis. We have identified a novel Prep1-interacting protein, p160 c-Myb binding protein (p160). p160 and Pbx1 compete for Prep1 in vitro, and p160 inhibits Prep1-dependent HoxB2 expression in retinoic acid-treated NT2-D1 cells. The N-terminal physiologically truncated form of pi 60, p67, binds the sequence 63LFPLL67 in the HR1 domain of Prep1. Mutation of both L63 and L66 impairs the binding of Prep1 to both p160/p67 and Pbx1. The sequences required to bind Prep1 are mainly located in residues 51 to 151. Immunofluorescence colocalization and coimmunoprecipitation of endogenous p160 and Prep1 are induced by ActD, which translocates p160 from the nucleolus to the nucleoplasm. These data therefore show that p160 is a novel regulator of Prep1-Pbx1 transcriptional activity. Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Díaz, V., Mori, S., Longobardi, E., Menendez, G., Ferrai, C., Keough, R., et al. (2007). p160 Myb-binding protein interacts with Prep1 and inhibits its transcriptional activity. MOLECULAR AND CELLULAR BIOLOGY, 27(22), 7981-7990 [10.1128/MCB.01290-07].

p160 Myb-binding protein interacts with Prep1 and inhibits its transcriptional activity

MORI, SILVIA
Secondo
;
2007

Abstract

Prep1 is known to interact in vivo with Pbx1 to regulate development and organogenesis. We have identified a novel Prep1-interacting protein, p160 c-Myb binding protein (p160). p160 and Pbx1 compete for Prep1 in vitro, and p160 inhibits Prep1-dependent HoxB2 expression in retinoic acid-treated NT2-D1 cells. The N-terminal physiologically truncated form of pi 60, p67, binds the sequence 63LFPLL67 in the HR1 domain of Prep1. Mutation of both L63 and L66 impairs the binding of Prep1 to both p160/p67 and Pbx1. The sequences required to bind Prep1 are mainly located in residues 51 to 151. Immunofluorescence colocalization and coimmunoprecipitation of endogenous p160 and Prep1 are induced by ActD, which translocates p160 from the nucleolus to the nucleoplasm. These data therefore show that p160 is a novel regulator of Prep1-Pbx1 transcriptional activity. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Articolo in rivista - Articolo scientifico
Amino Acid Sequence; Animals; Carrier Proteins; Cell Nucleus; Homeodomain Proteins; Mice; Molecular Sequence Data; NIH 3T3 Cells; Nuclear Proteins; Sequence Alignment; Transcription Factors; Gene Expression Regulation; Transcription, Genetic; Molecular Biology; Genetics; Cell Biology
English
2007
27
22
7981
7990
none
Díaz, V., Mori, S., Longobardi, E., Menendez, G., Ferrai, C., Keough, R., et al. (2007). p160 Myb-binding protein interacts with Prep1 and inhibits its transcriptional activity. MOLECULAR AND CELLULAR BIOLOGY, 27(22), 7981-7990 [10.1128/MCB.01290-07].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/105730
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