A new method of three-dimensional (3-D) analysis of sclerotised structures of monogenoids was performed by processing z-series images using 3D-Doctor. Z-series were obtained from Gomori's trichrome-stained specimens of marine and freshwater monogenoids under laser scanning confocal fluorescence microscopy. Measurements obtained from 3-D images were then compared with those from 2-D images taken from both flattened and unflattened specimens. Data comparison demonstrated that 3-D morphometry allowed avoidance of over-estimation due to deformation and the reduction of errors associated with different spatial orientations. Moreover, study of 3-D images permitted observation of morphological details that are not detectable in 2-D representations. © 2006 Australian Society for Parasitology Inc.

Galli, P., Strona, G., Villa, A., Benzoni, F., Stefani, F., Doglia, S., et al. (2007). Two-dimensional versus three-dimensional morphometry of monogenoidean sclerites. INTERNATIONAL JOURNAL FOR PARASITOLOGY, 37(3-4), 449-456 [10.1016/j.ijpara.2006.11.017].

Two-dimensional versus three-dimensional morphometry of monogenoidean sclerites

Galli, P;Villa, AM;Benzoni, F;Doglia, SM;
2007

Abstract

A new method of three-dimensional (3-D) analysis of sclerotised structures of monogenoids was performed by processing z-series images using 3D-Doctor. Z-series were obtained from Gomori's trichrome-stained specimens of marine and freshwater monogenoids under laser scanning confocal fluorescence microscopy. Measurements obtained from 3-D images were then compared with those from 2-D images taken from both flattened and unflattened specimens. Data comparison demonstrated that 3-D morphometry allowed avoidance of over-estimation due to deformation and the reduction of errors associated with different spatial orientations. Moreover, study of 3-D images permitted observation of morphological details that are not detectable in 2-D representations. © 2006 Australian Society for Parasitology Inc.
Articolo in rivista - Articolo scientifico
Monogenoidea, 3D
English
2007
37
3-4
449
456
reserved
Galli, P., Strona, G., Villa, A., Benzoni, F., Stefani, F., Doglia, S., et al. (2007). Two-dimensional versus three-dimensional morphometry of monogenoidean sclerites. INTERNATIONAL JOURNAL FOR PARASITOLOGY, 37(3-4), 449-456 [10.1016/j.ijpara.2006.11.017].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/1053
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