Rapid and accurate identification of enterococci at the species level is an essential task in clinical microbiology since these organisms have emerged as one of the leading causes of nosocomial infections worldwide. Vibrational spectroscopic techniques (infrared [IR] and Raman) could provide potential alternatives to conventional typing methods, because they are fast, easy to perform, and economical. We present a comparative study using phenotypic, genotypic, and vibrational spectroscopic techniques for typing a collection of 18 Enterococcus strains comprising six different species. Classification of the bacteria by Fourier transform (FT)-IR spectroscopy in combination with hierarchical cluster analysis revealed discrepancies for certain strains when compared with results obtained from automated phenotypic systems, such as API and MicroScan. Further diagnostic evaluation using genotypic methods-i.e., PCR of the species specific ligase and glycopeptide resistance genes, which is limited to the identification of only four Enterococcus species and 16S RNA sequencing, the "gold standard" for identification of enterococci-confirmed the results obtained by the FT-IR classification. These results were later reproduced by three different laboratories, using confocal Raman microspectroscopy, FT-IR attenuated total reflectance spectroscopy, and FT-IR microspectroscopy, demonstrating the discriminative capacity and the reproducibility of the technique. It is concluded that vibrational spectroscopic techniques have great potential as routine methods in clinical microbiology

Kirschner, C., Maquelin, K., Pina, P., Ngo Thi, N., Choo Smith, L., Sockalingum, G., et al. (2001). Classification and identification of enterococci: a comparative phenotypic, genotypic, and vibrational spectroscopic study. JOURNAL OF CLINICAL MICROBIOLOGY, 39(5), 1763-1770 [10.1128/JCM.39.5.1763-1770.2001].

Classification and identification of enterococci: a comparative phenotypic, genotypic, and vibrational spectroscopic study

Ami, D;Doglia, SM;
2001

Abstract

Rapid and accurate identification of enterococci at the species level is an essential task in clinical microbiology since these organisms have emerged as one of the leading causes of nosocomial infections worldwide. Vibrational spectroscopic techniques (infrared [IR] and Raman) could provide potential alternatives to conventional typing methods, because they are fast, easy to perform, and economical. We present a comparative study using phenotypic, genotypic, and vibrational spectroscopic techniques for typing a collection of 18 Enterococcus strains comprising six different species. Classification of the bacteria by Fourier transform (FT)-IR spectroscopy in combination with hierarchical cluster analysis revealed discrepancies for certain strains when compared with results obtained from automated phenotypic systems, such as API and MicroScan. Further diagnostic evaluation using genotypic methods-i.e., PCR of the species specific ligase and glycopeptide resistance genes, which is limited to the identification of only four Enterococcus species and 16S RNA sequencing, the "gold standard" for identification of enterococci-confirmed the results obtained by the FT-IR classification. These results were later reproduced by three different laboratories, using confocal Raman microspectroscopy, FT-IR attenuated total reflectance spectroscopy, and FT-IR microspectroscopy, demonstrating the discriminative capacity and the reproducibility of the technique. It is concluded that vibrational spectroscopic techniques have great potential as routine methods in clinical microbiology
Articolo in rivista - Articolo scientifico
FT-IR spectroscopy and microspectrosopy, Raman spectroscopy, identification of Enterococci
English
2001
39
5
1763
1770
none
Kirschner, C., Maquelin, K., Pina, P., Ngo Thi, N., Choo Smith, L., Sockalingum, G., et al. (2001). Classification and identification of enterococci: a comparative phenotypic, genotypic, and vibrational spectroscopic study. JOURNAL OF CLINICAL MICROBIOLOGY, 39(5), 1763-1770 [10.1128/JCM.39.5.1763-1770.2001].
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/1047
Citazioni
  • Scopus 239
  • ???jsp.display-item.citation.isi??? 222
Social impact