An artificial aggrecan-like proteoglycan has been designed and synthesized in vitro. At variance with natural proteoglycans, whose glycosaminoglycan chains are always O-linked via a tetrasaccharide bridge to the serine residues of a specific protein core, the present structure consists of chondroitin-6-sulfate chains directly bound to the lysine and hydroxylysine residues of a collagen molecule backbone. The resulting macromolecule has been characterized by histochemistry, atomic force microscopy and FTIR. The number of variables involved (e.g., length and type of the collagen backbone, glycosaminoglycan species, sulfation type and pattern, molecular weight, number and length of side chains, etc.) makes possible to conceive an almost endless variety of artificial proteoglycans, each precisely tailored to a specific functional role. In addition to their use as biomaterials, glycated collagens interact with cells in complex ways and a previous study has already shown the ability of a glycated collagen to redirect fibroblastoma cells from proliferation to differentiation. The research is still underway.

Raspanti, M., Caravà, E., Sgambato, A., Natalello, A., Russo, L., Cipolla, L. (2016). The collaggrecan: Synthesis and visualization of an artificial proteoglycan. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 86, 65-70 [10.1016/j.ijbiomac.2016.01.051].

The collaggrecan: Synthesis and visualization of an artificial proteoglycan

SGAMBATO, ANTONELLA;NATALELLO, ANTONINO;RUSSO, LAURA;CIPOLLA, LAURA FRANCESCA
2016

Abstract

An artificial aggrecan-like proteoglycan has been designed and synthesized in vitro. At variance with natural proteoglycans, whose glycosaminoglycan chains are always O-linked via a tetrasaccharide bridge to the serine residues of a specific protein core, the present structure consists of chondroitin-6-sulfate chains directly bound to the lysine and hydroxylysine residues of a collagen molecule backbone. The resulting macromolecule has been characterized by histochemistry, atomic force microscopy and FTIR. The number of variables involved (e.g., length and type of the collagen backbone, glycosaminoglycan species, sulfation type and pattern, molecular weight, number and length of side chains, etc.) makes possible to conceive an almost endless variety of artificial proteoglycans, each precisely tailored to a specific functional role. In addition to their use as biomaterials, glycated collagens interact with cells in complex ways and a previous study has already shown the ability of a glycated collagen to redirect fibroblastoma cells from proliferation to differentiation. The research is still underway.
Articolo in rivista - Articolo scientifico
Collagen; Glycation; Proteoglycans;
Collagen; Proteoglycans; Glycation
English
2016
86
65
70
none
Raspanti, M., Caravà, E., Sgambato, A., Natalello, A., Russo, L., Cipolla, L. (2016). The collaggrecan: Synthesis and visualization of an artificial proteoglycan. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 86, 65-70 [10.1016/j.ijbiomac.2016.01.051].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/102113
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