Phloem, a plant tissue responsible for long-distance molecular transport, harbours specific junctions, sieve areas, between the conducting cells. To date, little is known about the molecular framework related to the biogenesis of these sieve areas. Here we identify mutations at the CHER1/AtCTL1 locus of Arabidopsis thaliana. The mutations cause several phenotypic abnormalities, including reduced pore density and altered pore structure in the sieve areas associated with impaired phloem function. CHER1 encodes a member of a poorly characterized choline transporter-like protein family in plants and animals. We show that CHER1 facilitates choline transport, localizes to the trans-Golgi network, and during cytokinesis is associated with the phragmoplast. Consistent with its function in the elaboration of the sieve areas, CHER1 has a sustained, polar localization in the forming sieve plates. Our results indicate that the regulation of choline levels is crucial for phloem development and conductivity in plants. © 2014 Macmillan Publishers Limited. All rights reserved.

Dettmer, J., Ursache, R., Campilho, A., Miyashima, S., Belevich, I., O'Regan, S., et al. (2014). CHOLINE TRANSPORTER-LIKE1 is required for sieve plate development to mediate long-distance cell-to-cell communication. NATURE COMMUNICATIONS, 5, 4276 [10.1038/ncomms5276].

CHOLINE TRANSPORTER-LIKE1 is required for sieve plate development to mediate long-distance cell-to-cell communication

BEVERINA, LUCA;PAPAGNI, ANTONIO;
2014

Abstract

Phloem, a plant tissue responsible for long-distance molecular transport, harbours specific junctions, sieve areas, between the conducting cells. To date, little is known about the molecular framework related to the biogenesis of these sieve areas. Here we identify mutations at the CHER1/AtCTL1 locus of Arabidopsis thaliana. The mutations cause several phenotypic abnormalities, including reduced pore density and altered pore structure in the sieve areas associated with impaired phloem function. CHER1 encodes a member of a poorly characterized choline transporter-like protein family in plants and animals. We show that CHER1 facilitates choline transport, localizes to the trans-Golgi network, and during cytokinesis is associated with the phragmoplast. Consistent with its function in the elaboration of the sieve areas, CHER1 has a sustained, polar localization in the forming sieve plates. Our results indicate that the regulation of choline levels is crucial for phloem development and conductivity in plants. © 2014 Macmillan Publishers Limited. All rights reserved.
Articolo in rivista - Articolo scientifico
Biochemistry, Genetics and Molecular Biology (all); Chemistry (all); Physics and Astronomy (all)
English
4276
Dettmer, J., Ursache, R., Campilho, A., Miyashima, S., Belevich, I., O'Regan, S., et al. (2014). CHOLINE TRANSPORTER-LIKE1 is required for sieve plate development to mediate long-distance cell-to-cell communication. NATURE COMMUNICATIONS, 5, 4276 [10.1038/ncomms5276].
Dettmer, J; Ursache, R; Campilho, A; Miyashima, S; Belevich, I; O'Regan, S; Mullendore, D; Yadav, S; Lanz, C; Beverina, L; Papagni, A; Schneeberger, K; Weigel, D; Stierhof, Y; Moritz, T; Knoblauch, M; Jokitalo, E; Helariutta, Y
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/100476
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