The helicase domain of the hepatitis C virus (HCV) protein NS3 is a major immunodominant region that elicits a strong and specific immune response in the majority of HCV infections. Use of NS3 helicase-derived antigens in immunometric diagnostic assays is a mainstay in detection of HCV infections. However, thermal instability of NS3 helicase-related polypeptides is a common and severe issue hampering reproducibility, performances and shelf-life of HCV diagnostic immunoassays. In this work, a novel NS3 helicase-derived antigen is presented, with a sensible improvement in thermal stability and diagnostic performances over the currently used NS3 helicase-related antigens.

(2009). Study of the hepatitis C virus NS3 helicase domain for application in a chemiluminescent immunoassay. (Tesi di dottorato, Università degli Studi di Milano-Bicocca, 2009).

Study of the hepatitis C virus NS3 helicase domain for application in a chemiluminescent immunoassay

REBUZZINI, GABRIELE
2009

Abstract

The helicase domain of the hepatitis C virus (HCV) protein NS3 is a major immunodominant region that elicits a strong and specific immune response in the majority of HCV infections. Use of NS3 helicase-derived antigens in immunometric diagnostic assays is a mainstay in detection of HCV infections. However, thermal instability of NS3 helicase-related polypeptides is a common and severe issue hampering reproducibility, performances and shelf-life of HCV diagnostic immunoassays. In this work, a novel NS3 helicase-derived antigen is presented, with a sensible improvement in thermal stability and diagnostic performances over the currently used NS3 helicase-related antigens.
PORRO, DANILO
DAL CORSO, ANDREA
HCV,NS3,helicase,hepatitis,virus,immunoassay,c33,thermal,stability,antigen
CHIM/11 - CHIMICA E BIOTECNOLOGIA DELLE FERMENTAZIONI
English
Scuola di dottorato di Scienze
BIOTECNOLOGIE INDUSTRIALI - 15R
22
2008/2009
(2009). Study of the hepatitis C virus NS3 helicase domain for application in a chemiluminescent immunoassay. (Tesi di dottorato, Università degli Studi di Milano-Bicocca, 2009).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/7477
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