Three main researches have been employed for the implementation of a protocol analysis for the characterization and quantification of the lignin fraction in the particulate matter at the concentration matrix level; the implementation of different methods of analysis of the toxic interesting pollutants, Oxy-PAHs; Nitro-PAHs and the Bisphenol A, that together with the large set of performed analysis, allowed the characterization of some PM fractions in relation with Indoor and Outdoor concentrations, human exposure and Urban – Rural – Remote sites composition. At last an in silica method was developed for the research of the proteins involved in the interaction with the pollutants of interest, optimized on Bisphenol A because of its history and recent interaction study with the Nuclear Receptors. From the involved pathway the Blood Serine Proteases are used to test the accuracy and reproducibility of obtained Autodock4.0 and Dock4.0 data. The method results useful for research on the biological mechanism of action in relation with both matrix concentrations and in vivo and in vitro studies. The data predicted will be confirmed by NMR analysis. The newest docking program gives more and more reproducible data, accurate and empirically shaped on the domain problem, at last the experimental data had to confirm or not confirm the predictions.

(2009). Speciation of particulate matter's organic fraction and its mechanis of action on human health. (Tesi di dottorato, Università degli Studi di Milano-Bicocca, 2009).

Speciation of particulate matter's organic fraction and its mechanis of action on human health

LAZZATI, ZELDA
2009-12-11

Abstract

Three main researches have been employed for the implementation of a protocol analysis for the characterization and quantification of the lignin fraction in the particulate matter at the concentration matrix level; the implementation of different methods of analysis of the toxic interesting pollutants, Oxy-PAHs; Nitro-PAHs and the Bisphenol A, that together with the large set of performed analysis, allowed the characterization of some PM fractions in relation with Indoor and Outdoor concentrations, human exposure and Urban – Rural – Remote sites composition. At last an in silica method was developed for the research of the proteins involved in the interaction with the pollutants of interest, optimized on Bisphenol A because of its history and recent interaction study with the Nuclear Receptors. From the involved pathway the Blood Serine Proteases are used to test the accuracy and reproducibility of obtained Autodock4.0 and Dock4.0 data. The method results useful for research on the biological mechanism of action in relation with both matrix concentrations and in vivo and in vitro studies. The data predicted will be confirmed by NMR analysis. The newest docking program gives more and more reproducible data, accurate and empirically shaped on the domain problem, at last the experimental data had to confirm or not confirm the predictions.
BOLZACCHINI, EZIO
Liquid Chromatography Mass Spectrometry; Gas Chromatography Mass Spectrometry; Molecular Docking; Oxy-PAHs; Nitro-PAHs; Bisphenol A; Lignin Polymeric Fraction; Particulate Matter; Zeisel Technique; Biological Mechanism of Action.
CHIM/01 - CHIMICA ANALITICA
English
Scuola di dottorato di Scienze
SCIENZE CHIMICHE - 18R
21
2007/2008
(2009). Speciation of particulate matter's organic fraction and its mechanis of action on human health. (Tesi di dottorato, Università degli Studi di Milano-Bicocca, 2009).
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/7466
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