Mass spectrometry imaging (MSI) is an emerging technology that is capable of mapping various biomolecules within their native spatial context, and performing spatial multiomics on formalin-fixed paraffin-embedded (FFPE) tissues may further increase the molecular characterization of pathological states. Here we present a novel workflow which enables the sequential MSI of lipids, N- glycans, and tryptic peptides on a single FFPE tissue section and highlight the enhanced molecular characterization that is offered by combining the multiple spatial omics data sets. In murine brain and clear cell renal cell carcinoma (ccRCC) tissue, the three molecular levels provided complementary information and characterized differ- ent histological regions. Moreover, when the spatial omics data was integrated, the different histopathological regions of the ccRCC tissue could be better discriminated with respect to the imaging data set of any single omics class. Taken together, these promising findings demonstrate the capability to more comprehensively map the molecular complexity within pathological tissue.

Denti, V., Capitoli, G., Piga, I., Clerici, F., Pagani, L., Criscuolo, L., et al. (2023). Erratum: Spatial Multiomics of Lipids, N-Glycans, and Tryptic Peptides on a Single FFPE Tissue Section (Journal of Proteome Research (2022) 21: 11 (2798−2809) DOI: 10.1021/acs.jproteome.2c00601) [Altro] [10.1021/acs.jproteome.3c00217].

Erratum: Spatial Multiomics of Lipids, N-Glycans, and Tryptic Peptides on a Single FFPE Tissue Section (Journal of Proteome Research (2022) 21: 11 (2798−2809) DOI: 10.1021/acs.jproteome.2c00601)

Denti V.
Primo
;
Capitoli G.
Secondo
;
Piga I.;Clerici F.;Pagani L.;Bindi G.;Chinello C.;Paglia G.;Magni F.;Smith A.
Ultimo
2023

Abstract

Mass spectrometry imaging (MSI) is an emerging technology that is capable of mapping various biomolecules within their native spatial context, and performing spatial multiomics on formalin-fixed paraffin-embedded (FFPE) tissues may further increase the molecular characterization of pathological states. Here we present a novel workflow which enables the sequential MSI of lipids, N- glycans, and tryptic peptides on a single FFPE tissue section and highlight the enhanced molecular characterization that is offered by combining the multiple spatial omics data sets. In murine brain and clear cell renal cell carcinoma (ccRCC) tissue, the three molecular levels provided complementary information and characterized differ- ent histological regions. Moreover, when the spatial omics data was integrated, the different histopathological regions of the ccRCC tissue could be better discriminated with respect to the imaging data set of any single omics class. Taken together, these promising findings demonstrate the capability to more comprehensively map the molecular complexity within pathological tissue.
Altro
Erratum; Correction
English
2023
This paper was originally not requested by the authors for consideration in the submissions for the Methods for Omics Research 2023 Special Issue, https://pubs.acs.org/toc/jprobs/22/5. However, Guest Editors of this Special Issue later identified this paper as a contribution worth mention related to the Special Issue. Therefore, the paper has been linked to the Special Issue. Scopus ID 2-s2.0-85159609906; WOS ID WOS:000983271300001; PubMed ID 37144619
Denti, V., Capitoli, G., Piga, I., Clerici, F., Pagani, L., Criscuolo, L., et al. (2023). Erratum: Spatial Multiomics of Lipids, N-Glycans, and Tryptic Peptides on a Single FFPE Tissue Section (Journal of Proteome Research (2022) 21: 11 (2798−2809) DOI: 10.1021/acs.jproteome.2c00601) [Altro] [10.1021/acs.jproteome.3c00217].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/417682
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