Targeted Protein Silencing (TPS) is an elegant approach to investigate protein function and its role in the cellular landscape, overcoming limitations of genetic perturbation strategies. In contrast to CRISPR/Cas9 and RNA interference, these systems act in a reversible manner and reduce off-target effects. Several TPS have been developed and wisely improved, including compartment delocalization tools and protein degradation systems. In this review, we focus on Anchor-Away, deGradFP, auxin inducible degron (AID) and dTAG technologies, and discuss their recent applications and advances. Finally, we propose Nano-Grad, a novel nanobody-based protein degradation tool to specifically proteolyze endogenous tag-free target protein.

Prozzillo, Y., Fattorini, G., Virginia Santopietro, M., Suglia, L., Ruggiero, A., Ferreri, D., et al. (2020). Targeted-Protein Silencing Tools: Overview and Future Perspectives [Altro] [10.20944/preprints202011.0061.v1].

Targeted-Protein Silencing Tools: Overview and Future Perspectives

Giovanni Messina
Ultimo
2020

Abstract

Targeted Protein Silencing (TPS) is an elegant approach to investigate protein function and its role in the cellular landscape, overcoming limitations of genetic perturbation strategies. In contrast to CRISPR/Cas9 and RNA interference, these systems act in a reversible manner and reduce off-target effects. Several TPS have been developed and wisely improved, including compartment delocalization tools and protein degradation systems. In this review, we focus on Anchor-Away, deGradFP, auxin inducible degron (AID) and dTAG technologies, and discuss their recent applications and advances. Finally, we propose Nano-Grad, a novel nanobody-based protein degradation tool to specifically proteolyze endogenous tag-free target protein.
Altro
Targeted Protein Silencing (TPS); Targeted Protein Degradation (TPD); dTAG; FKBP12; von Hippel-Lindau (VHL); degron; deGradFP; Anchor-Away; Nanobody; Nano-Grad
English
2-nov-2020
2020
Preprint
Prozzillo, Y., Fattorini, G., Virginia Santopietro, M., Suglia, L., Ruggiero, A., Ferreri, D., et al. (2020). Targeted-Protein Silencing Tools: Overview and Future Perspectives [Altro] [10.20944/preprints202011.0061.v1].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/414876
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