The present invention relates to fungal enzymes, more in particular of polyphenoloxidases (PPOs), and is based on a newly discovered enzymatic activity of a class of PPOs, i.e. de-methylation of R-substituted mono- or di-methoxyphenolsuch as present in lignin, lignin derived compounds and/or in lignocellulosic biomass. This newly discovered enzymatic activity renders these enzymes highly suitable for a plethora of applications in industry. Provided herein are methods of demethylation, processes to increase the reactivity of lignin or lignin-comprising biomass, processes of conversion lignin or lignin comprising biomass to value added products, processes for degrading and/or modifying (hemi-)cellulose in a hemicellulose-comprising substrate, and expression vectors, host cells and liquids, pastes or solid formulations and compositions for use in the demethylation method of the invention.

J Koetsier, M., Visser, J., L Iancu, S., A Kabel, M., Frommhagen, M., Gruppen, H., et al. (2016)POLYPEPTIDES HAVING DEMETHYLATING ACTIVITY. . Brevetto No. 20190002846.

POLYPEPTIDES HAVING DEMETHYLATING ACTIVITY

H Lange;
2016

Abstract

The present invention relates to fungal enzymes, more in particular of polyphenoloxidases (PPOs), and is based on a newly discovered enzymatic activity of a class of PPOs, i.e. de-methylation of R-substituted mono- or di-methoxyphenolsuch as present in lignin, lignin derived compounds and/or in lignocellulosic biomass. This newly discovered enzymatic activity renders these enzymes highly suitable for a plethora of applications in industry. Provided herein are methods of demethylation, processes to increase the reactivity of lignin or lignin-comprising biomass, processes of conversion lignin or lignin comprising biomass to value added products, processes for degrading and/or modifying (hemi-)cellulose in a hemicellulose-comprising substrate, and expression vectors, host cells and liquids, pastes or solid formulations and compositions for use in the demethylation method of the invention.
Si
Lignin, enzymes, demethylation, activation.
Chemistry, Metallurgy
Rilevanza internazionale
20190002846
Altro titolare
J Koetsier, M; Visser, J; L Iancu, S; A Kabel, M; Frommhagen, M; Gruppen, H; Lange, H; Crestini, C; Benjelloun-Mlayah, B
J Koetsier, M., Visser, J., L Iancu, S., A Kabel, M., Frommhagen, M., Gruppen, H., et al. (2016)POLYPEPTIDES HAVING DEMETHYLATING ACTIVITY. . Brevetto No. 20190002846.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/323404
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