Despite the relevance of carbohydrates as cues in eliciting specific biological responses, the covalent surface modification of collagen-based matrices with small carbohydrate epitopes has been scarcely investigated. We report thereby the development of an efficient procedure for the chemoselective neoglycosylation of collagen matrices (patches) via a thiol-ene approach, between alkene-derived monosaccharides and the thiol-functionalized material surface. Synchrotron radiation-induced X-ray photoelectron spectroscopy (SR-XPS), Fourier transform-infrared (FT-IR), and enzyme-linked lectin assay (ELLA) confirmed the effectiveness of the collagen neoglycosylation. Preliminary biological evaluation in osteoarthritic models is reported. The proposed methodology can be extended to any thiolated surface for the development f smart biomaterials for innovative approaches in regenerative medicine

Russo, L., Battocchio, C., Secchi, V., Magnano, E., Nappini, S., Taraballi, F., et al. (2015). Thiol-ene mediated neoglycosylation of collagen patches: a preliminary study [Altro].

Thiol-ene mediated neoglycosylation of collagen patches: a preliminary study

RUSSO, LAURA
;
TARABALLI, FRANCESCA
;
GABRIELLI, LUCA
;
COMELLI, FRANCESCA
;
PAPAGNI, ANTONIO
;
COSTA, BARBARA SIMONA
;
NICOTRA, FRANCESCO
;
NATALELLO, ANTONINO
;
DOGLIA, SILVIA MARIA
;
CIPOLLA, LAURA FRANCESCA
Ultimo
2015

Abstract

Despite the relevance of carbohydrates as cues in eliciting specific biological responses, the covalent surface modification of collagen-based matrices with small carbohydrate epitopes has been scarcely investigated. We report thereby the development of an efficient procedure for the chemoselective neoglycosylation of collagen matrices (patches) via a thiol-ene approach, between alkene-derived monosaccharides and the thiol-functionalized material surface. Synchrotron radiation-induced X-ray photoelectron spectroscopy (SR-XPS), Fourier transform-infrared (FT-IR), and enzyme-linked lectin assay (ELLA) confirmed the effectiveness of the collagen neoglycosylation. Preliminary biological evaluation in osteoarthritic models is reported. The proposed methodology can be extended to any thiolated surface for the development f smart biomaterials for innovative approaches in regenerative medicine
Altro
Scientifica
collagen, biomaterials, regenerative medicine, osteoarthritis
English
"Thiol–ene Mediated Neoglycosylation of Collagen Patches: A Preliminary Study" handle 10281/50643 è stato pubblicato su Langmiur e ripubblicato in forma modificata su Global Medical Discovery.
Russo, L., Battocchio, C., Secchi, V., Magnano, E., Nappini, S., Taraballi, F., et al. (2015). Thiol-ene mediated neoglycosylation of collagen patches: a preliminary study [Altro].
Russo, L; Battocchio, C; Secchi, V; Magnano, E; Nappini, S; Taraballi, F; Gabrielli, L; Comelli, F; Papagni, A; Costa, B; Polzonetti, G; Nicotra, F; Natalello, A; Doglia, S; Cipolla, L
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/135157
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