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|Authors: ||GALIMBERTI, ANDREA|
|Internal tutor : ||CASIRAGHI, MAURIZIO|
|Title: ||DNA barcoding: a link between basic and applied science|
|Abstract: ||DNA barcoding is a recent and widely used molecular-based identification system that aims to identify biological specimens, and to assign them to a given species. However, DNA
barcoding is even more than this, and besides many practical uses, it can be considered the
core of an integrated taxonomic system, where bioinformatics plays a key role. Quite soon
since its development (in 2003) it became clear that DNA barcoding was suitable for two
different purposes: (i) the molecular identification of already described species and (ii) the
discovery of undescribed species (the so called ‘DNA taxonomy’). However, such a method
has generated a vast debate in the scientific community, which has been from the beginning,
deeply divided into pros and cons.
The main objective of this research project was to investigate the strength of coherence
reached in combining a standardized molecular methodology with classical biological
information (e.g. morphology, ecology, host specificity), toward the synthesis of an integrated
approach to taxonomy. In order to satisfy this requirement, nine case studies encompassing a
wide panel of taxa (i.e. animal, plant and environmental samples) subjected to different
taxonomic uncertainties or potentially dealing with economical, conservation or health
implications (e.g. food traceability, parasites infectiveness, etc.) have been investigated.
More than 500 hundreds biological samples were collected directly in the field or retrieved
from museum, herbariums or other institutional collections, allowing to create a synergic
network among different disciplines and research fields. Standardization in the collection and
processing of biological samples, as well as in the bioinformatic approaches used to manage
and analyse molecular data has been a fundamental point in the experimental workflow we
The results obtained with our analyses clearly showed that DNA barcoding represents a
powerful tool for taxonomy and it can act as an effective supporting tool for the traceability of
food products, for the diagnosis of endoparasites and for the characterization of
environmental biodiversity. Although some limitations arise from the incomplete coverage of
the existing diversity, the inherent characteristics of the molecular markers adopted as
barcodes and other factors, the method showed to be more flexible than expected.|
|Keywords: ||DNA barcoding; IOTUs; Biodiversity; DNA Taxonomy|
|MIUR Subject : ||Settore BIO/05 - Zoologia|
|ISO Language : ||eng|
|Issue Date: ||2011-01-27|
|Doctoral school: ||Scuola di dottorato di Scienze|
|Doctoral course: ||BIOLOGIA|
|Doctoral cycle: ||23|
|Academic year: ||2009/2010|
|Other Information: ||Il suddetto progetto di ricerca è stato condotto per quanto riguarda le analisi sperimentali presso il Laboratorio ZooPlantLab (Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano Bicocca) e ha portato alla pubblicazione dei seguenti articoli su riviste internazionali: 1.1 - Ferri E., Barbuto M., Bain O., Galimberti A., Uni S., Guerrero R., Fertè H., Bandi C., Martin C., Casiraghi M., 2009. Integrated taxonomy: traditional approach and DNA barcoding for the identification of filarioid worms and related parasites (Nematoda). Frontiers In Zoology Volume: 6 Article Number: 1.
1.2 - Barbuto M., Galimberti A., Ferri E., Labra M., Malandra R., Galli P., Casiraghi C., 2010. DNA barcoding reveals fraudulent substitutions in shark seafood products: the Italian case of “palombo” (Mustelus spp.). Food Research International Volume: 43 Issue: 1 Pages: 376-381.
1.3 - Casiraghi, M., Labra, M., Ferri, E., Galimberti, A., De Mattia, F., 2010. DNA barcoding: a six-question tour to improve users' awareness about the method. Briefing in Bioinformatics 11(4):440-453.
1.4. - Bruni, I., De Mattia, F., Galimberti, A., Galasso, G., Banfi, E., Casiraghi, M., Labra, M., 2010. Identification of poisonous plants by DNA barcoding approach. International Journal of Legal Medicine 124(6):595-603.
1.5. - Galimberti A., Crottini A., Boto A., Serra L., Liu Y., Yeung C., Xiaojun Y., Barbuto M., Casiraghi M..Toward a resolution of a taxonomic enigma: first genetic analyses of Paradoxornis webbianus and P. alphonsianus (Aves: Paradoxornithidae) from China and Italy. Molecular phylogenetics and Evolution 57: 1312-1318.|
|Citation: ||GALIMBERTI, A. (2011). DNA barcoding: a link between basic and applied science. (Tesi di dottorato, Università degli Studi di Milano-Bicocca, 2011).|
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