Protein kinase CK2 is a heterotetramer composed of two catalytic and two regulatory subunits. In Saccharomyces cerevisiae the catalytic subunits (¿ and ¿¿) are encoded by the CKA1, CKA2 genes. cka1¿cka2ts mutants arrest cell cycle in both G1 and G2/M at 37°C. Hence, it has been proposed that CK2 plays an important role in cell cycle progression and several cell cycle proteins have been reported to be CK2 substrates. We have previously shown that Sic1, the inhibitor of Clb5-Cdc28 complexes required for the G1/S transition, is a physiologically relevant CK2 substrate. Here we show that CK2 inactivation up-regulates Sic1 level resulting in severe down-regulation of Clb5-Cdc28 kinase activity. Concurrent inactivation of Sic1 and CK2 leads to accumulation of cells with a post-synthetic DNA content and short/elongated spindles, typical of cells arrested in mitosis. These findings indicate that Sic1 plays a major role during G1 arrest of CK2-inactivated cells

Tripodi, F., Zinzalla, V., Vanoni, M., Alberghina, L., Coccetti, P. (2007). In CK2 inactivated cells the cyclin dependent kinase inhibitor Sic1 is involved in cell-cycle arrest bifore the onset of S phase. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 359(4), 921-927 [10.1016/J.BBRC.2007.05.195].

In CK2 inactivated cells the cyclin dependent kinase inhibitor Sic1 is involved in cell-cycle arrest bifore the onset of S phase

TRIPODI, FARIDA;VANONI, MARCO ERCOLE;ALBERGHINA, LILIA;COCCETTI, PAOLA
2007

Abstract

Protein kinase CK2 is a heterotetramer composed of two catalytic and two regulatory subunits. In Saccharomyces cerevisiae the catalytic subunits (¿ and ¿¿) are encoded by the CKA1, CKA2 genes. cka1¿cka2ts mutants arrest cell cycle in both G1 and G2/M at 37°C. Hence, it has been proposed that CK2 plays an important role in cell cycle progression and several cell cycle proteins have been reported to be CK2 substrates. We have previously shown that Sic1, the inhibitor of Clb5-Cdc28 complexes required for the G1/S transition, is a physiologically relevant CK2 substrate. Here we show that CK2 inactivation up-regulates Sic1 level resulting in severe down-regulation of Clb5-Cdc28 kinase activity. Concurrent inactivation of Sic1 and CK2 leads to accumulation of cells with a post-synthetic DNA content and short/elongated spindles, typical of cells arrested in mitosis. These findings indicate that Sic1 plays a major role during G1 arrest of CK2-inactivated cells
Articolo in rivista - Articolo scientifico
Cell cycle, Saccharomyces cerevisiae, Cki, Sic1, CK2, G1/S transition
English
2007
359
4
921
927
none
Tripodi, F., Zinzalla, V., Vanoni, M., Alberghina, L., Coccetti, P. (2007). In CK2 inactivated cells the cyclin dependent kinase inhibitor Sic1 is involved in cell-cycle arrest bifore the onset of S phase. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 359(4), 921-927 [10.1016/J.BBRC.2007.05.195].
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/649
Citazioni
  • Scopus 23
  • ???jsp.display-item.citation.isi??? 23
Social impact