The results are discussed of a systematic investigation into the electromagnetic held (EMP) exposure consequences on human lymphocytes. These artificial fields have intensities comparable to the Earth's magnetic field, and are used for exposures up to 4 days. Different and complementary techniques are used to safely assess the consequences of EMFs on the cells; in particular, morphology, metabolism, and population dynamics are investigated. The recourse to ultramicroscopy, pressure monitoring in sealed bottles, atomic mass spectroscopy, and cytofluorimetry techniques give good insight into the EMF-induced changes. A statistically significant deviation of irradiated samples with respect to control samples is reported. A critical analysis and a survey of similar experiments reported in the literature led us to examine the experimental setup with attention to the geometry of the irradiation system. Yeast cells were used as a model system to statistically test the different steps in the overall procedure, thanks to information gathered during a radiobiology experiment performed at the Rutherford Appleton Laboratory. Finally, the role of different magnetic field detectors in the reproducibility of the experiments is carefully discussed.

Milani, M., Ballerini, M., Ferraro, L., Zabeo, M., Barberis, M., Cannone, M., et al. (2001). Magnetic field effects on human lymphocytes. ELECTRO- AND MAGNETOBIOLOGY, 20(1), 81-106 [10.1081/JBC-100103162].

Magnetic field effects on human lymphocytes

MILANI, MARZIALE;FERRARO, LORENZO;
2001

Abstract

The results are discussed of a systematic investigation into the electromagnetic held (EMP) exposure consequences on human lymphocytes. These artificial fields have intensities comparable to the Earth's magnetic field, and are used for exposures up to 4 days. Different and complementary techniques are used to safely assess the consequences of EMFs on the cells; in particular, morphology, metabolism, and population dynamics are investigated. The recourse to ultramicroscopy, pressure monitoring in sealed bottles, atomic mass spectroscopy, and cytofluorimetry techniques give good insight into the EMF-induced changes. A statistically significant deviation of irradiated samples with respect to control samples is reported. A critical analysis and a survey of similar experiments reported in the literature led us to examine the experimental setup with attention to the geometry of the irradiation system. Yeast cells were used as a model system to statistically test the different steps in the overall procedure, thanks to information gathered during a radiobiology experiment performed at the Rutherford Appleton Laboratory. Finally, the role of different magnetic field detectors in the reproducibility of the experiments is carefully discussed.
Articolo in rivista - Articolo scientifico
Magnetic field, human lymphocytes
English
2001
20
1
81
106
none
Milani, M., Ballerini, M., Ferraro, L., Zabeo, M., Barberis, M., Cannone, M., et al. (2001). Magnetic field effects on human lymphocytes. ELECTRO- AND MAGNETOBIOLOGY, 20(1), 81-106 [10.1081/JBC-100103162].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/4046
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