Green fluorescent protein (GFP) and its derivatives are the most widely used molecular reporters for live cell imagining. The development of organelle-specific fusion fluorescent proteins improves the labeling resolution to a higher level. Here we generate a R26 dual fluorescent protein reporter mouse, activated by Cre-mediated DNA recombination, labeling target cells with a chromatin-specific enhanced green fluorescence protein (EGFP) and a plasma membrane-anchored monomeric cherry fluorescent protein (mCherry). This dual labeling allows the visualization of mitotic events, cell shapes and intracellular vesicle behaviors. We expect this reporter mouse to have a wide application in developmental biology studies, transplantation experiments as well as cancer/stem cell lineage tracing.

Chen, Y., Tsai, M., Yang, T., Ku, A., Huang, K., Huang, C., et al. (2012). R26R-GR: A Cre-Activable Dual Fluorescent Protein Reporter Mouse. PLOS ONE, 7(9), e46171 [10.1371/journal.pone.0046171].

R26R-GR: A Cre-Activable Dual Fluorescent Protein Reporter Mouse

FAVARO, REBECCA;NICOLIS, SILVIA KIRSTEN;
2012

Abstract

Green fluorescent protein (GFP) and its derivatives are the most widely used molecular reporters for live cell imagining. The development of organelle-specific fusion fluorescent proteins improves the labeling resolution to a higher level. Here we generate a R26 dual fluorescent protein reporter mouse, activated by Cre-mediated DNA recombination, labeling target cells with a chromatin-specific enhanced green fluorescence protein (EGFP) and a plasma membrane-anchored monomeric cherry fluorescent protein (mCherry). This dual labeling allows the visualization of mitotic events, cell shapes and intracellular vesicle behaviors. We expect this reporter mouse to have a wide application in developmental biology studies, transplantation experiments as well as cancer/stem cell lineage tracing.
Articolo in rivista - Articolo scientifico
reporter gene, transgene
English
2012
7
9
e46171
none
Chen, Y., Tsai, M., Yang, T., Ku, A., Huang, K., Huang, C., et al. (2012). R26R-GR: A Cre-Activable Dual Fluorescent Protein Reporter Mouse. PLOS ONE, 7(9), e46171 [10.1371/journal.pone.0046171].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/39373
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