We investigated the cell association and intracellular pathway to the nucleus of complexes formed between DNA and cationic lipid DOTAP (lipoplexes) or cationic polymer polyethylenimine (polyplexes). Flow cytometry and confocal microscope analysis showed that lipoplexes presented higher affinity for cell membrane than polyplexes. Electron microscopy demonstrated that both types of complexes followed an endocytic pathway and were metabolized but did not enter the nucleus. However, by in situ PCR and FISH it was possible to show that the plasmid localized to the nuclei, indicating that DNA must be dissociated from the vectors to be delivered to the nucleus. Our results identify a different behaviour in the interaction of polyplexes and lipoplexes with the cell, indicating that a combination of advantageous properties of the two kinds of cationic molecules could further ameliorate efficiency of nonviral gene transfer vectors. © Conese et al.

Conese, M., Biffi, A., Dina, G., Marziliano, N., Villa, A. (2009). Comparison between cationic polymer and lipid in plasmidic DNA delivery to the cell nucleus. THE OPEN GENE THERAPY JOURNAL, 2(1), 21-28 [10.2174/1875037000902010021].

Comparison between cationic polymer and lipid in plasmidic DNA delivery to the cell nucleus

VILLA, ANTONELLO
2009

Abstract

We investigated the cell association and intracellular pathway to the nucleus of complexes formed between DNA and cationic lipid DOTAP (lipoplexes) or cationic polymer polyethylenimine (polyplexes). Flow cytometry and confocal microscope analysis showed that lipoplexes presented higher affinity for cell membrane than polyplexes. Electron microscopy demonstrated that both types of complexes followed an endocytic pathway and were metabolized but did not enter the nucleus. However, by in situ PCR and FISH it was possible to show that the plasmid localized to the nuclei, indicating that DNA must be dissociated from the vectors to be delivered to the nucleus. Our results identify a different behaviour in the interaction of polyplexes and lipoplexes with the cell, indicating that a combination of advantageous properties of the two kinds of cationic molecules could further ameliorate efficiency of nonviral gene transfer vectors. © Conese et al.
Articolo in rivista - Articolo scientifico
gene transfer vectors
English
2009
2
1
21
28
none
Conese, M., Biffi, A., Dina, G., Marziliano, N., Villa, A. (2009). Comparison between cationic polymer and lipid in plasmidic DNA delivery to the cell nucleus. THE OPEN GENE THERAPY JOURNAL, 2(1), 21-28 [10.2174/1875037000902010021].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/39199
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