Transformed Saccharomyces cerevisiae cells overexpressing the Escherichia coli LacZ gene and the transcriptional activator GAL4, release in the external medium a fraction (from 2 to 10%) of the total beta-galactosidase activity (Porro et al., 1992b). It is known that this abnormal release of a cytoplasmic protein is related to a partial cell lysis of the yeast population, which is likely to be caused by the overexpression of the transcriptional activator GAL4. In the present paper we have characterized the GAL4-induced cell lysis phenomenon. The expression of the GAL4 gene causes morphological modifications and alteration of the cell size distribution. The cell lysis is independent of the expression of the heterologous LacZ gene and occurs in a specific subpopulation of cells (the parent cells) independently of the genealogical age, growth phase conditions and cell cycle progression. Lysis is preceded by a loss of the plasma membrane integrity as indicated by the uptake of ethidium bromide in unfixed cells. Computer analysis of simulated protein distributions indicates that cell lysis takes place in a sizeable aliquot (about 50%) of the parent cells, therefore profoundly altering the age structure of the population.

Martegani, E., Brambilla, L., Porro, D., Ranzi, B., Alberghina, L. (1993). Alteration of cell population structure due to cell lysis in Saccharomyces cerevisiae cells overexpressing the GAL4 gene. YEAST, 9(6), 575-582 [10.1002/yea.320090603].

Alteration of cell population structure due to cell lysis in Saccharomyces cerevisiae cells overexpressing the GAL4 gene

MARTEGANI, ENZO;BRAMBILLA, LUCA GIUSEPPE;PORRO, DANILO;ALBERGHINA, LILIA
1993

Abstract

Transformed Saccharomyces cerevisiae cells overexpressing the Escherichia coli LacZ gene and the transcriptional activator GAL4, release in the external medium a fraction (from 2 to 10%) of the total beta-galactosidase activity (Porro et al., 1992b). It is known that this abnormal release of a cytoplasmic protein is related to a partial cell lysis of the yeast population, which is likely to be caused by the overexpression of the transcriptional activator GAL4. In the present paper we have characterized the GAL4-induced cell lysis phenomenon. The expression of the GAL4 gene causes morphological modifications and alteration of the cell size distribution. The cell lysis is independent of the expression of the heterologous LacZ gene and occurs in a specific subpopulation of cells (the parent cells) independently of the genealogical age, growth phase conditions and cell cycle progression. Lysis is preceded by a loss of the plasma membrane integrity as indicated by the uptake of ethidium bromide in unfixed cells. Computer analysis of simulated protein distributions indicates that cell lysis takes place in a sizeable aliquot (about 50%) of the parent cells, therefore profoundly altering the age structure of the population.
Articolo in rivista - Articolo scientifico
Gene Expression Regulation, Fungal; Cell Fractionation; beta-Galactosidase; Transcription Factors; Genes, Fungal; Transformation, Genetic; DNA-Binding Proteins; Saccharomyces cerevisiae Proteins; Flow Cytometry; Models, Biological; Fungal Proteins; Saccharomyces cerevisiae
English
giu-1993
9
6
575
582
none
Martegani, E., Brambilla, L., Porro, D., Ranzi, B., Alberghina, L. (1993). Alteration of cell population structure due to cell lysis in Saccharomyces cerevisiae cells overexpressing the GAL4 gene. YEAST, 9(6), 575-582 [10.1002/yea.320090603].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/34274
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