The exciting aptitude of sugars to form high-affinity interactions with various biological entities exploiting multivalency has led to the assessment of the potential of conjugating mono- and oligosaccharides to nanopartieles to investigate the biorecognition events and interfere with biological processes involving carbohydrates. Since a critical step in these studies is the fine control on the presentation of the saccharide molecules, reliable methods to check their presence and, possibly, their arrangement on the surface of nanopartieles are urgently required. In this chapter, we provide an overview of the most common analytical techniques useful to characterize the saccharidic portion of glycoconjugate nanomaterials. Among them, HRMAS NMR looks particularly promising, as it can resolve the structures of the immobilized ligands and may provide information on their orientation on the nanoparticle surface
Morasso, C., Verderio, P., Colombo, M., Prosperi, D. (2011). Strategies for the characterization of the saccharidic moiety in composite nanoparticles. In X. Huang, J.J. Barchi (a cura di), Petite and Sweet: Glyco-nanotechnology as a Bridge to New Medicines (pp. 69-89). Washington, DC : American Chemical Society [10.1021/bk-2011-1091.ch005].
Strategies for the characterization of the saccharidic moiety in composite nanoparticles
COLOMBO, MIRIAM;PROSPERI, DAVIDE
2011
Abstract
The exciting aptitude of sugars to form high-affinity interactions with various biological entities exploiting multivalency has led to the assessment of the potential of conjugating mono- and oligosaccharides to nanopartieles to investigate the biorecognition events and interfere with biological processes involving carbohydrates. Since a critical step in these studies is the fine control on the presentation of the saccharide molecules, reliable methods to check their presence and, possibly, their arrangement on the surface of nanopartieles are urgently required. In this chapter, we provide an overview of the most common analytical techniques useful to characterize the saccharidic portion of glycoconjugate nanomaterials. Among them, HRMAS NMR looks particularly promising, as it can resolve the structures of the immobilized ligands and may provide information on their orientation on the nanoparticle surface
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