Corneal Pachymetry and Endothelial Microscopy by Slit-Lamp

A slit-lamp biomicroscope Visionix VX75 has been equipped with a high- resolution digital sensor. A specular reflection technique at an angular magnifica- tion of 36 performed by the slit-lamp biomicroscope is used to develop a proce- dure to (i) measure the thickness of the human cornea by measuring the distance between the two reflections of its anterior and posterior surfaces and (ii) capture suitable images for morphometric analyses of the corneal endothelium’s cell mosaic. The examples of morphometric analysis of these images are reported. The biases due to the dioptric power of the anterior surface of the cornea, the oblique obser- vation, and the asymmetry of the digital biomicroscope are discussed. These biases can be corrected by a specific calibration.