In bone tissue engineering, bioglass coating of titanium (Ti) scaffolds has drawn attention as a method to improve osteointegration and implant fixation. In this in vitro study, bioactive glass layers with an approximate thickness of 1 mm were deposited at 2008C onto a three-dimensional Ti-6Al-4V scaffold using a radio frequency (r.f.) magnetron sputtering system. After incubation with SAOS-2 human osteoblasts, in comparison with the uncoated scaffolds, the bioglass-coated scaffolds showed a twofold increase in cell proliferation ( p<0.05) up to 68.4106, and enhanced the deposition of extracellular matrix components such as decorin, fibronectin, osteocalcin, osteonectin, osteopontin, and type-I and -III collagens ( p<0.05). Calcium deposition was twofold greater on the bioglass-coated scaffolds ( p<0.05). The immunofluorescence related to the preceding bone matrix proteins and calcium showed their colocalization to the cell-rich areas. Alkaline phosphatase activity increased twofold ( p<0.001) and its protein content was threefold higher with respect to the uncoated sample. Quantitative reverse transcriptase–polymerase chain reaction analysis revealed upregulated transcription specific for type-I collagen and osteopontin ( p<0.001). All together, these results demonstrate that the bioglass coating of the three-dimensional Ti scaffolds by the r.f. magnetron sputtering technique determines an in vitro increase of the bone matrix elaboration and may potentially have a clinical benefit
Saino, E., Maliardi, V., Quartarone, E., Fassina, L., Benedetti, L., Cusella De Angelis, M., et al. (2010). In Vitro Enhancement of SAOS-2 Cell Calcified Matrix Deposition onto Radio Frequency Magnetron Sputtered Bioglass-Coated Titanium Scaffolds. TISSUE ENGINEERING, PART A, 16(3), 995-1008 [10.1089/ten.tea.2009.0051].
In Vitro Enhancement of SAOS-2 Cell Calcified Matrix Deposition onto Radio Frequency Magnetron Sputtered Bioglass-Coated Titanium Scaffolds
Mustarelli, P;
2010
Abstract
In bone tissue engineering, bioglass coating of titanium (Ti) scaffolds has drawn attention as a method to improve osteointegration and implant fixation. In this in vitro study, bioactive glass layers with an approximate thickness of 1 mm were deposited at 2008C onto a three-dimensional Ti-6Al-4V scaffold using a radio frequency (r.f.) magnetron sputtering system. After incubation with SAOS-2 human osteoblasts, in comparison with the uncoated scaffolds, the bioglass-coated scaffolds showed a twofold increase in cell proliferation ( p<0.05) up to 68.4106, and enhanced the deposition of extracellular matrix components such as decorin, fibronectin, osteocalcin, osteonectin, osteopontin, and type-I and -III collagens ( p<0.05). Calcium deposition was twofold greater on the bioglass-coated scaffolds ( p<0.05). The immunofluorescence related to the preceding bone matrix proteins and calcium showed their colocalization to the cell-rich areas. Alkaline phosphatase activity increased twofold ( p<0.001) and its protein content was threefold higher with respect to the uncoated sample. Quantitative reverse transcriptase–polymerase chain reaction analysis revealed upregulated transcription specific for type-I collagen and osteopontin ( p<0.001). All together, these results demonstrate that the bioglass coating of the three-dimensional Ti scaffolds by the r.f. magnetron sputtering technique determines an in vitro increase of the bone matrix elaboration and may potentially have a clinical benefitFile | Dimensione | Formato | |
---|---|---|---|
Tissue Engineering 2010_printed.pdf
Solo gestori archivio
Dimensione
472.47 kB
Formato
Adobe PDF
|
472.47 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.