Fluorescence correlation spectroscopy (FCS) was first developed for biophysical studies in analogy with photon scattering correlation spectroscopy. Although it is mainly devoted to the study of freely diffusing particles, FCS is actually able to discern between different kinds of motions, such as diffusion, anomalous diffusion, or drift motions. The frontier application of FCS nowadays is in medical studies both within cells and on the cell membranes, and in the investigation of single molecules in solid matrices. In this field, FCS originated also image correlation spectroscopy methods. The whole field can be unified under the name of fluorescence fluctuation spectroscopy (FFS). We present here a short review of the theoretical bases of FFS under a unified vision and discuss some applications to the study of dynamics of nanoparticles in cells and to the investigation of the photodynamics of immobilized dyes.

Collini, M., D'Alfonso, L., Caccia, M., Sironi, L., Panziaca, M., Chirico, G., et al. (2011). Invitro- Invivo fluctuation spectroscopies. In A. Diaspro (a cura di), Optical Fluorescence Microscopy: From the Spectral to the Nano Dimension (pp. 165-181). Springer Berlin Heidelberg [10.1007/978-3-642-15175-0_10].

Invitro- Invivo fluctuation spectroscopies

COLLINI, MADDALENA;D'ALFONSO, LAURA;SIRONI, LAURA;CHIRICO, GIUSEPPE
;
RIVOLTA, ILARIA;MISEROCCHI, GIUSEPPE ANDREA
2011

Abstract

Fluorescence correlation spectroscopy (FCS) was first developed for biophysical studies in analogy with photon scattering correlation spectroscopy. Although it is mainly devoted to the study of freely diffusing particles, FCS is actually able to discern between different kinds of motions, such as diffusion, anomalous diffusion, or drift motions. The frontier application of FCS nowadays is in medical studies both within cells and on the cell membranes, and in the investigation of single molecules in solid matrices. In this field, FCS originated also image correlation spectroscopy methods. The whole field can be unified under the name of fluorescence fluctuation spectroscopy (FFS). We present here a short review of the theoretical bases of FFS under a unified vision and discuss some applications to the study of dynamics of nanoparticles in cells and to the investigation of the photodynamics of immobilized dyes.
Capitolo o saggio
microscopy; fluorescence; correlation spectroscopy
English
Optical Fluorescence Microscopy: From the Spectral to the Nano Dimension
Diaspro, A
2011
978-3-642-15174-3
Springer Berlin Heidelberg
165
181
Collini, M., D'Alfonso, L., Caccia, M., Sironi, L., Panziaca, M., Chirico, G., et al. (2011). Invitro- Invivo fluctuation spectroscopies. In A. Diaspro (a cura di), Optical Fluorescence Microscopy: From the Spectral to the Nano Dimension (pp. 165-181). Springer Berlin Heidelberg [10.1007/978-3-642-15175-0_10].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/21249
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