Background: Ventricular fibrillation (VF) is a major cause of sudden cardiac death. In some cases clinical investigations fail to identify the underlying cause and the event is classified as idiopathic (IVF). Sincemutations in arrhythmia-associated genes frequently determine arrhythmia susceptibility, screening for diseasepredisposing variants could improve IVF diagnostics. Methods and results: The study included 76 Finnish and Italian patients with a mean age of 31.2 years at the time of the VF event, collected between the years 1996–2016 and diagnosed with idiopathic, out-of-hospital VF. Using whole-exome sequencing (WES) and next-generation sequencing (NGS) approaches, we aimed to identify genetic variants potentially contributing to the life-threatening arrhythmias of these patients. Combining the results from the two study populations, we identified pathogenic or likely pathogenic variants residing in the RYR2, CACNA1C and DSP genes in 7 patients (9%).Most of them(5, 71%)were found in the RYR2 gene, associated with catecholaminergic polymorphic ventricular tachycardia (CPVT). These genetic findings prompted clinical investigations leading to disease reclassification. Additionally, in 9 patients (11.8%) we detected 10 novel or extremely rare (MAF b 0.005%) variants that were classified as of unknown significance (VUS). Conclusion: The results of our study suggest that a subset of patients originally diagnosed with IVFmay carry clinically-relevant variants in genes associated with cardiac channelopathies and cardiomyopathies. Although misclassification of other cardiac channelopathies as IVF appears rare, our findings indicate that the possibility of CPVT as the underlying disease entity should be carefully evaluated in IVF patients

Leinonen, J., Crotti, L., Djupsjöbacka, A., Castelletti, S., Junna, N., Ghidoni, A., et al. (2018). The genetics underlying idiopathic ventricular fibrillation: A special role for catecholaminergic polymorphic ventricular tachycardia?. INTERNATIONAL JOURNAL OF CARDIOLOGY, 250, 139-145 [10.1016/j.ijcard.2017.10.016].

The genetics underlying idiopathic ventricular fibrillation: A special role for catecholaminergic polymorphic ventricular tachycardia?

Crotti, L
Co-primo
;
2018

Abstract

Background: Ventricular fibrillation (VF) is a major cause of sudden cardiac death. In some cases clinical investigations fail to identify the underlying cause and the event is classified as idiopathic (IVF). Sincemutations in arrhythmia-associated genes frequently determine arrhythmia susceptibility, screening for diseasepredisposing variants could improve IVF diagnostics. Methods and results: The study included 76 Finnish and Italian patients with a mean age of 31.2 years at the time of the VF event, collected between the years 1996–2016 and diagnosed with idiopathic, out-of-hospital VF. Using whole-exome sequencing (WES) and next-generation sequencing (NGS) approaches, we aimed to identify genetic variants potentially contributing to the life-threatening arrhythmias of these patients. Combining the results from the two study populations, we identified pathogenic or likely pathogenic variants residing in the RYR2, CACNA1C and DSP genes in 7 patients (9%).Most of them(5, 71%)were found in the RYR2 gene, associated with catecholaminergic polymorphic ventricular tachycardia (CPVT). These genetic findings prompted clinical investigations leading to disease reclassification. Additionally, in 9 patients (11.8%) we detected 10 novel or extremely rare (MAF b 0.005%) variants that were classified as of unknown significance (VUS). Conclusion: The results of our study suggest that a subset of patients originally diagnosed with IVFmay carry clinically-relevant variants in genes associated with cardiac channelopathies and cardiomyopathies. Although misclassification of other cardiac channelopathies as IVF appears rare, our findings indicate that the possibility of CPVT as the underlying disease entity should be carefully evaluated in IVF patients
Articolo in rivista - Articolo scientifico
Idiopathic ventricular fibrillation, Genetics, Catecholaminergic polymorphic ventricular tachycardia, RYR2, Genetic testing
English
2018
250
139
145
reserved
Leinonen, J., Crotti, L., Djupsjöbacka, A., Castelletti, S., Junna, N., Ghidoni, A., et al. (2018). The genetics underlying idiopathic ventricular fibrillation: A special role for catecholaminergic polymorphic ventricular tachycardia?. INTERNATIONAL JOURNAL OF CARDIOLOGY, 250, 139-145 [10.1016/j.ijcard.2017.10.016].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/189266
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