The binding of 125I-labeled substance P (SP) to rat brain cortex membranes has been studied under control conditions and in the presence of ethanol. The binding of SP at low concentrations (20-1000 pM) gave two components, one with a KD value of 80 pM and another one with a KD of 500 pM. The higher-affinity component is due to NK1 receptors, as confirmed by the inhibition of the SP binding by the rodent NK1 specific agonist [Sar9 Met(O2)11]SP. Ethanol (1.7 mM) added to the binding assays inhibited by more than 50% the specific binding at a very low SP concentration (20 pM); however, it had no effect at SP concentrations ranging from 50 to 120 pM. This suggests a decrease by ethanol of the affinity of SP to the NK1 receptors involved in this binding component. The ethanol effect disappeared at [EtOH] < or = 0.17 mM

Scarrone, S., Cappelli, A., Cupello, A., Matarrese, M., Moresco, R., Fazio, F. (2003). Ethanol Inhibits the Binding of Substance P to Rat Brain Cortex NK1 Receptors. NEUROCHEMICAL RESEARCH, 28(8), 1159-1162 [10.1023/A:1024216109472].

Ethanol Inhibits the Binding of Substance P to Rat Brain Cortex NK1 Receptors

Moresco, RM;Fazio, F.
2003

Abstract

The binding of 125I-labeled substance P (SP) to rat brain cortex membranes has been studied under control conditions and in the presence of ethanol. The binding of SP at low concentrations (20-1000 pM) gave two components, one with a KD value of 80 pM and another one with a KD of 500 pM. The higher-affinity component is due to NK1 receptors, as confirmed by the inhibition of the SP binding by the rodent NK1 specific agonist [Sar9 Met(O2)11]SP. Ethanol (1.7 mM) added to the binding assays inhibited by more than 50% the specific binding at a very low SP concentration (20 pM); however, it had no effect at SP concentrations ranging from 50 to 120 pM. This suggests a decrease by ethanol of the affinity of SP to the NK1 receptors involved in this binding component. The ethanol effect disappeared at [EtOH] < or = 0.17 mM
Articolo in rivista - Articolo scientifico
ethanol, substance P, brain
English
2003
28
8
1159
1162
none
Scarrone, S., Cappelli, A., Cupello, A., Matarrese, M., Moresco, R., Fazio, F. (2003). Ethanol Inhibits the Binding of Substance P to Rat Brain Cortex NK1 Receptors. NEUROCHEMICAL RESEARCH, 28(8), 1159-1162 [10.1023/A:1024216109472].
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/1871
Citazioni
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
Social impact