We report the analysis of the fluorescence intensity fluctuations of single proteins of a GFP mutant, GFPmut2, embedded in a polyelectrolyte nanocapsule adsorbed on thin conductive layers. Our results, based on single molecule fluorescence spectroscopy, indicate that the fluorescence blinking dynamics of GFP is strongly dependent on the bulk conductivity of the metal layer substrate, on the distance from the conductive surfaces and on the amplitude of the voltage applied to the poly-electrolyte layers. These findings suggest that fluorescence blinking itself might be employed as a reporter signal in nano-bio-technology applications. (c) 2006 Elsevier B.V. All rights reserved.
Cannone, F., Milani, R., Chirico, G., Diaspro, A., Krol, S., Campanini, B. (2007). Voltage regulation of single green fluorescent protein mutants. BIOPHYSICAL CHEMISTRY, 125(2-3), 368-374 [10.1016/j.bpc.2006.09.012].
Voltage regulation of single green fluorescent protein mutants
MILANI, ROBERTO;CHIRICO, GIUSEPPE;
2007
Abstract
We report the analysis of the fluorescence intensity fluctuations of single proteins of a GFP mutant, GFPmut2, embedded in a polyelectrolyte nanocapsule adsorbed on thin conductive layers. Our results, based on single molecule fluorescence spectroscopy, indicate that the fluorescence blinking dynamics of GFP is strongly dependent on the bulk conductivity of the metal layer substrate, on the distance from the conductive surfaces and on the amplitude of the voltage applied to the poly-electrolyte layers. These findings suggest that fluorescence blinking itself might be employed as a reporter signal in nano-bio-technology applications. (c) 2006 Elsevier B.V. All rights reserved.
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