Differential localisation of nPKC delta during cell cycle progression

nPKCdelta is a phospholipid-dependent and calcium-independent PKC isoform, whose over expression in BL6T murine melanoma cells, modifies their proliferative and metastatic potential in vivo, We focus here on the possible relationship between the subcellular localisation of nPKCdelta and distinct phase of the cell cycle. Our findings show a dynamic localisation of nPKCdelta in dependence of the phase of the cell cycle. Actually, this isoform is preferentially localised to the cytoplasm in serum-starved cells, shifting to the nucleus during the S-phase and becoming peri-nuclear, associated to the Golgi apparatus, in G2-M phase. Therefore, taken together our findings demonstrate that the subcellular localisation of nPKCdelta changes dynamically during the cell cycle in dependence of the requirement of the enzyme at a particular place of the cell. (C) 2002 Elsevier Science (USA). All rights reserved.