Flow-cytometric analysis was employed to investigate growth dynamics of a yeast cell population immobilised in an insolubilised gelatin gel by means of the quantitative determination of the average protein content per cell. This analysis was carried out on both the immobilised cell population considered as a whole and the subpopulations colonising the gelatin matrix at different depths. The results show that growth of the gelatin-immobilised yeast population was affected by the existence of a gradient of nutrient concentrations through the matrix and are in agreement with the unsteady-state diffusion model employed for the description of glucose transfer in the gel

de Alteriis, E., Porro, D., Romano, V., Parascandola, P. (2001). Relation between growth dynamics and diffusional limitations in Saccharomyces cerevisiae cells growing as entrapped in an insolubilised gelatin gel. FEMS MICROBIOLOGY LETTERS, 195(2), 245-251 [10.1016/S0378-1097(01)00017-9].

Relation between growth dynamics and diffusional limitations in Saccharomyces cerevisiae cells growing as entrapped in an insolubilised gelatin gel

PORRO, DANILO;
2001

Abstract

Flow-cytometric analysis was employed to investigate growth dynamics of a yeast cell population immobilised in an insolubilised gelatin gel by means of the quantitative determination of the average protein content per cell. This analysis was carried out on both the immobilised cell population considered as a whole and the subpopulations colonising the gelatin matrix at different depths. The results show that growth of the gelatin-immobilised yeast population was affected by the existence of a gradient of nutrient concentrations through the matrix and are in agreement with the unsteady-state diffusion model employed for the description of glucose transfer in the gel
Articolo in rivista - Articolo scientifico
Saccharomyces cerevisiae, Flow citometry, growth dynamics
English
2001
195
2
245
251
none
de Alteriis, E., Porro, D., Romano, V., Parascandola, P. (2001). Relation between growth dynamics and diffusional limitations in Saccharomyces cerevisiae cells growing as entrapped in an insolubilised gelatin gel. FEMS MICROBIOLOGY LETTERS, 195(2), 245-251 [10.1016/S0378-1097(01)00017-9].
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/17175
Citazioni
  • Scopus 6
  • ???jsp.display-item.citation.isi??? 7
Social impact