Glomerulonephritis (GNs) are one of the most frequent causes of chronic kidney disease (CKD), a renal condition that often leads to end-stage renal failure, and a careful assessment of these diseases is essential for prognostic and therapeutic purposes. The application of MALDI-MSI directly on bioptic renal tissue represents a new stimulating perspective and facilitates the detection of specific proteomic indicators that are directly correlated with the pathological alterations that occur within the glomeruli during the development of glomerulonephritis. Here, we describe the standard workflow for the MALDI-MSI analysis of clinical fresh-frozen and FFPE renal biopsies and highlight how the obtained molecular information, when combined with histology, can be used to detect specific protein markers of GNs.

Smith, A., Galli, M., L'Imperio, V., Pagni, F., Magni, F. (2017). MALDI-MS imaging in the study of glomerulonephritis. In L. Cole (a cura di), Imaging Mass Spectrometry (pp. 85-94). Humana Press Inc. [10.1007/978-1-4939-7051-3_9].

MALDI-MS imaging in the study of glomerulonephritis

SMITH, ANDREW JAMES
;
GALLI, MANUEL
Secondo
;
L'imperio, V;PAGNI, FABIO
Penultimo
;
MAGNI, FULVIO
Ultimo
2017

Abstract

Glomerulonephritis (GNs) are one of the most frequent causes of chronic kidney disease (CKD), a renal condition that often leads to end-stage renal failure, and a careful assessment of these diseases is essential for prognostic and therapeutic purposes. The application of MALDI-MSI directly on bioptic renal tissue represents a new stimulating perspective and facilitates the detection of specific proteomic indicators that are directly correlated with the pathological alterations that occur within the glomeruli during the development of glomerulonephritis. Here, we describe the standard workflow for the MALDI-MSI analysis of clinical fresh-frozen and FFPE renal biopsies and highlight how the obtained molecular information, when combined with histology, can be used to detect specific protein markers of GNs.
Capitolo o saggio
Glomerulonephritis; Kidney; MALDI-imaging; Proteomics; Molecular Biology; Genetics
English
Imaging Mass Spectrometry
Cole, L
2017
978-1-4939-7050-6
1618
Humana Press Inc.
85
94
Smith, A., Galli, M., L'Imperio, V., Pagni, F., Magni, F. (2017). MALDI-MS imaging in the study of glomerulonephritis. In L. Cole (a cura di), Imaging Mass Spectrometry (pp. 85-94). Humana Press Inc. [10.1007/978-1-4939-7051-3_9].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/167961
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