Recombinant DNA (rDNA) technologies (genetic, protein, and metabolic engineering) allow the production of a wide range of peptides, proteins, and biochemicals from naturally nonproducing cells. This technology, now approx 25 yr old, is becoming one of the most important technologies developed in the 20th century.Pharmaceutical products and industrial enzymes were the first biotech products on the world market made by means of rDNA. Despite important advances regarding rDNA applications in mammalian cells, yeasts still represent attractive hosts for the production of heterologous proteins. In this review we summarize the advantages and limitations of the main and promising yeast hosts.
Porro, D., Mattanovich, D. (2004). Recombinant protein production in yeasts. In P. Balbás, A. Lorence (a cura di), Recombinant Gene Expression Reviews and Protocols (pp. 241-258). Humana Press, Inc. [10.1385/1-59259-774-2:241].
Recombinant protein production in yeasts
Porro, D;
2004
Abstract
Recombinant DNA (rDNA) technologies (genetic, protein, and metabolic engineering) allow the production of a wide range of peptides, proteins, and biochemicals from naturally nonproducing cells. This technology, now approx 25 yr old, is becoming one of the most important technologies developed in the 20th century.Pharmaceutical products and industrial enzymes were the first biotech products on the world market made by means of rDNA. Despite important advances regarding rDNA applications in mammalian cells, yeasts still represent attractive hosts for the production of heterologous proteins. In this review we summarize the advantages and limitations of the main and promising yeast hosts.
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