Germinal centers are short-lived microanatomical compartments with essential roles in adaptive immunity. These lymphoid structures can be identified in secondary lymphoid organs using both flow cytometry and immunohistological analyses, but only the latter provides useful architectural and spatial information. Here we describe how to use immunofluorescence and immunohistochemistry with specific antibodies to precisely highlight the cellular and architectural features of germinal centers, both in human and mouse secondary lymphoid organs, and to study their normal development and disturbance in disease.

Dominguez Sola, D., Cattoretti, G. (2017). Analysis of the germinal center reaction in tissue sections. In D. Calado (a cura di), Germinal Centers : Methods and Protocols (pp. 1-20). Humana Press Inc. [10.1007/978-1-4939-7095-7_1].

Analysis of the germinal center reaction in tissue sections

CATTORETTI, GIORGIO
Ultimo
2017

Abstract

Germinal centers are short-lived microanatomical compartments with essential roles in adaptive immunity. These lymphoid structures can be identified in secondary lymphoid organs using both flow cytometry and immunohistological analyses, but only the latter provides useful architectural and spatial information. Here we describe how to use immunofluorescence and immunohistochemistry with specific antibodies to precisely highlight the cellular and architectural features of germinal centers, both in human and mouse secondary lymphoid organs, and to study their normal development and disturbance in disease.
Capitolo o saggio
Antigen retrieval; Epitope; Formalin-fixed paraffin-embedded sections; Frozen sections; Immunofluorescence; Immunohistochemistry; Molecular Biology; Genetics
English
Germinal Centers : Methods and Protocols
Calado, D
2017
978-1-4939-7094-0
1623
Humana Press Inc.
1
20
Dominguez Sola, D., Cattoretti, G. (2017). Analysis of the germinal center reaction in tissue sections. In D. Calado (a cura di), Germinal Centers : Methods and Protocols (pp. 1-20). Humana Press Inc. [10.1007/978-1-4939-7095-7_1].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/163023
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